肾细胞癌中survivin基因的表达及其与细胞凋亡的关系

目的:探讨在肾细胞癌中survivin基因的表达及其与细胞凋亡的关系。方法:应用免疫组织化学技术和末端脱氧核苷酸转移酶介导的X-dUTP缺口末端标记法(TUNEL)检测41例肾细胞癌中survivin基因的表达与细胞凋亡情况。结果:41例肾细胞癌survivin阳性率为56.1%(23/41),9例正常肾组织survivin无表达,差别有显著意义(P<0.01)。Survivin表达与肾细胞癌病理类型无明显相关(P>0.05),与肾细胞癌病理分期和病理分级明显相关(P<0.05)。Survivin表达与调亡指数呈负相关(n=12,r=-0.58)(P<0.01)。结论:Survivin基因与肾细胞癌病程发展、恶性程度明显相关,对细胞凋亡有抑制作用,在肾癌的发生和发展中起重要作用。

Expression of survivin gene and its relationship to apoptosis in renal cell cancer

Objective To study the expression of survivin gene and its relationship to apoptosis in renal cell cancer (RCC).Methods Expression of survivin gene and apoptosis were assessed by immunohistochemistry staining and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling methods, respectively. Results In 41 cases of renal cell cancer, positive rate for survivin was 56.1%,it was significantly higher than normal control group(0%)(P< 0.01). There was no relationship between survivin and histologic subtype (P >0.05). However, survivin had relationship with the development of pathological stage and grade of RCC(P< 0.05). There was an inverse correlation between survivin labeling index and apoptosis index(n=12,r=-0.58)(P< 0.01). Conclusions survivin had relationship with biological behavior of RCC. Survivin is negatively correlated with apoptosis. Survivin could play a critical role in the development of RCC.