Indirect immunofluorescent antibody diagnosis of herpes simplex with upper tarsal and corneal scrapings |
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| Authors: | I R Schwab V K Raju J McClung |
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| Affiliation: | 1. School of Economics and Business Administration, Chongqing University, Chongqing 400044, PR China;2. Research Institute of Business Analytics and Supply Chain Management, College of Management, Shenzhen University, Shenzhen 518060, PR China;3. Research Center of Business Administration and Economic Development, Chongqing University, Chongqing 400044, PR China;1. Centre for Health, Law and Emerging Technologies (HeLEX), Faculty of Law, University of Oxford, Oxford, United Kingdom;2. HeLEX Melbourne, Melbourne Law School, The University of Melbourne, Carlton, VIC, Australia;3. Center for Genomic Health, Charles R. Bronfman Institute for Personalized Medicine, Icahn School of Medicine at Mount Sinai, Manhattan, NY, United States;4. Health Economics Research Centre (HERC), Nuffield Department of Population Health, University of Oxford, Oxford, United Kingdom;5. SingHealth Duke-NUS Institute of Precision Medicine (PRISM), Singapore, Singapore;6. Melbourne Genomics Health Alliance, Parkville, VIC, Australia;7. Faculty of Medicine, Dentistry and Health Sciences, The University of Melbourne, Melbourne, VIC, Australia;8. University of Exeter Medical School, Royal Devon & Exeter Hospital, Exeter, United Kingdom;9. Department of Biomedical Ethics and Public Policy, Graduate School of Medicine, Osaka University, Suita, Japan;10. National Center for Tumour Diseases (NCT), German Cancer Research Center (DKFZ), Heidelberg, Germany;11. Egenis Centre for the Study of the Life Sciences, University of Exeter, Exeter, United Kingdom;12. Center for Medical Ethics and Health Policy, Baylor College of Medicine, Houston, TX, United States;13. Estonian Genome Center, University of Tartu, Tartu, Estonia;15. Centre for Biomedical Ethics and Law, Katholieke Universiteit Leuven, Leuven, Belgium;p. Victorian Clinical Genetics Services, Murdoch Children’s Research Institute, Royal Children''s Hospital, Parkville, VIC, Australia |
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| Abstract: | Indirect immunofluorescent techniques were used to diagnose active herpes simplex virus ocular infections in 84 patient observations (41 with ocular lesions suspicious clinically for herpes simplex and 43 with lesions suspicious clinically for other ocular inflammatory conditions). We found indirect immunofluorescent antibody techniques to have a high sensitivity (97%) and specificity (73%) when compared to herpes simplex virus cultures. Similarly, we found the sensitivity (98%) and specificity (77%) of indirect immunofluorescent antibody techniques to be high when compared to the clinical diagnosis of herpes simplex viral infection. Significantly, there were no false negative tests by indirect immunofluorescent techniques. Both corneal and upper tarsal scrapings by indirect immunofluorescence were used and the upper tarsal scrapings were an excellent source of cells exhibiting herpes simplex virus antigens. All cases in which corneal scrapings were positive by indirect immunofluorescence for herpes simplex ere also positive by upper tarsal scrapings, although the converse was not true. |
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| Keywords: | corneal scrapings herpes simplex indirect immunofluorescence upper tarsal scrapings |
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