Emergence in Cx knockout mice of a diverse cytotoxic T lymphocyte repertoire that recognizes a single peptide from the immunoglobulin constant x light chain region |
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Authors: | Dominique Rueff-Juy Pierre Sanchez Mathias Faure Anne-Marie Drapier Pierre-Andr Cazenave |
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Institution: | Dominique Rueff-Juy,Pierre Sanchez,Mathias Faure,Anne-Marie Drapier,Pierre-André Cazenave |
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Abstract: | Allotype- or idiotype-specific CD4+ T cells have been reported to recognize immunoglobulin (Ig) peptides presented by class II molecules. In contrast, few data are available concerning the generation of Ig peptide-specific CD8+ T cells. We have therefore investigated whether T-depleted spleen cells from Ig x light chain-expressing 129/Sv mice (129x+/+) could induce, in Cx knockout mice (129 x?/?), the generation of Ig constant x light chain region (Cx)-specific cytotoxic T lymphocytes (CTL). The determination of TCRβ chain expressed by nine CTL clones, together with the use of a library of overlapping peptides spanning the whole Cx sequence, show that the B cells from x+/+ mice are able to elicit in Cx knockout mice, the emergence of a diverse CTL repertoire that recognizes one single Cx peptide presented by the H-2Kb class I molecule. In addition, these data support the notion that B cells are able to process and present on their class I molecules, peptides generated from their own x light chains. |
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Keywords: | Repertoire Cytotoxic T lymphocyte Cx knockout mice Peptide B lymphocyte |
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