辣木叶提取物对便秘小鼠润肠通便的作用及机制研究

目的 探究辣木叶Moringaoleifera醇提物和水提物对便秘小鼠的通便作用及机制.方法 昆明种小鼠随机分为对照组、模型组、酚酞(0.05 g/kg)组以及辣木叶醇提物低、中、高剂量(0.5、1.0、2.0g/kg)组和辣木叶水提物低、中、高剂量(0.5、1.0、2.0 g/kg)组,每组10只.通过失水燥结法建立...

Effect and mechanism of Moringa oleifera leaves extract on moistening intestines and purgation in constipated mice

Objective To investigate the cathartic effect and mechanism of water and ethanol extract from Lamu (Moringa oleifera) leaves on mice model with constipation. Methods Kunming mice were randomly divided into control group, model group, phenolphthalein (0.05 g/kg) group, low-, medium-, high-dose of ethanol extract from M. oleifera leaves (0.5, 1.0, 2.0 g/kg) group, and low-, medium-, high-dose of water extract from M. oleifera leaves (0.5, 1.0, 2.0 g/kg) group, with 10 mice in each group. A mouse constipation model was established by dehydration and dryness method, and drugs were given to intervene, the effects of alcohol extract and water extract of M. oleifera leaves on time of first defecation, fecal water content, number of defecation within 6 h, gastric emptying rate, and advancement rate of contents of small intestine in constipated mice were investigated. Hematoxylin-eosin (HE) staining was used to investigate the effects of alcohol and water extracts of M. oleifera leaves on pathological changes of small intestine tissue of constipated mice; ELISA kits were used to investigate the effects of extracts and water extracts of M. oleifera leaves on levels of gastrin, motilin, endothelin and nitric oxide in serum of constipated mice; Immunohistochemical methods were used to investigate the effects of alcohol extracts and water extracts of M. oleifera leaves on expressions of gastrin, motilin, endothelin and nitric oxide in small intestine of constipated mice. Results Alcohol extract and water extract of M. oleifera leaves significantly shorten the time of first defecation in mice (P < 0.01), increased the water content of feces (P < 0.05), increased the number of defecation in 6 h (P < 0.05), increased gastric emptying rate and small intestine content advance rate (P < 0.05, 0.01), up-regulated the secretions and expressions of gastrin, motilin, endothelin in serum and small intestine tissue (P < 0.05, 0.01), down-regulated the secretion and expression of nitric oxide in serum and small intestine tissue (P < 0.05, 0.01), reduced the injury of small intestine villi. Conclusion Alcohol extract and water extract of M. oleifera leaves can improve constipation in mice and achieve the effect of moistening intestines and purgation. Its mechanism may be related to regulating the level of gastrointestinal hormones and promoting intestinal motility in mice.