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The properties of the multicopy suppressor of the ogdl mutation in yeast
Authors:Yvetta Gbelsk  Daniela Mockov iakov  Jozef Timko  Július &#x;ubík
Institution:Yvetta Gbelská,Daniela Mockovčiaková,Jozef Timko,Július Šubík
Abstract:The 8.1 kb chromosomal fragment partially suppressing the ogdl mutation in Saccharomyces cerevisiae has been cloned. The molecular analysis revealed that its suppressor gene codes for a natural glutamine tRNACAG and maps on chromosome XIII in the upstream region of the URA10 gene. The multicopy plasmids containing this tRNA gene also suppressed the standard trpl-l amber mutation and conferred the sensitivity of yeast cells to paromomycin and increased temperature.
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