The properties of the multicopy suppressor of the ogdl mutation in yeast |
| |
Authors: | Yvetta Gbelsk Daniela Mockov
iakov Jozef Timko Július ubík |
| |
Institution: | Yvetta Gbelská,Daniela Mockovčiaková,Jozef Timko,Július Šubík |
| |
Abstract: | The 8.1 kb chromosomal fragment partially suppressing the ogdl mutation in Saccharomyces cerevisiae has been cloned. The molecular analysis revealed that its suppressor gene codes for a natural glutamine tRNACAG and maps on chromosome XIII in the upstream region of the URA10 gene. The multicopy plasmids containing this tRNA gene also suppressed the standard trpl-l amber mutation and conferred the sensitivity of yeast cells to paromomycin and increased temperature. |
| |
Keywords: | |
|
|