Interleukin-10 inhibits IgE-mediated nitric oxide synthase induction and cytokine synthesis in normal human keratinocytes |
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Authors: | Pierre-Andr Bcherel Liliane Le Goff Sandra Ktorza Fateh Ouaaz Jean-Michel Mencia-Huerta Bernard Dugas Patrice Debr M Djavad Mossalayi Michel Arock |
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Institution: | Pierre-André Bécherel,Liliane Le Goff,Sandra Ktorza,Fateh Ouaaz,Jean-Michel Mencia-Huerta,Bernard Dugas,Patrice Debré,M. Djavad Mossalayi,Michel Arock |
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Abstract: | Human keratinocytes (HK) generate nitric oxide (NO) and proinflammatory mediators following activation with either IgE/anti-IgE immune complexes or a combination of lipopolysaccharide (LPS) and interferon-γ (IFN-γ). Recently, interleukin-10 (IL-10) has been shown to down-regulate various inflammatory responses and to be secreted by lymphocytes and dendritic cells during skin inflammatory reactions. We show here that IL-10 down-regulates the production of tumor necrosis factor (TNF)-α and IL-6 by activated HK. Also, induction of inducible nitric oxide synthase (iNOS) expression in HK by IgE/anti-IgE or LPS/IFN-γ is significantly reduced by the addition of IL-10. This effect is dose dependent and correlates with reduction of iNOS mRNA production and enzyme level. Therefore, IL-10 down-regulates NO-mediated HK inflammatory responses and may thus participate in the regulation of the skin immune network. |
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Keywords: | Keratinocyte Nitric oxide Interleukin-10 IgE Tumor necrosis factor-α |
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