| 大肠杆菌感染对大鼠肺组织β-防御素-2基因表达的负相调节作用 |
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| 引用本文： | 陈新年,黄宁,吴琦,王伯瑶. 大肠杆菌感染对大鼠肺组织β-防御素-2基因表达的负相调节作用[J]. 中国病理生理杂志, 2002, 18(1): 83-85 |
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| 作者姓名： | 陈新年 黄宁 吴琦 王伯瑶 |
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| 作者单位： | 四川大学华西医学中心感染免疫研究室, 病理生理教研室, 四川成都 610041 |
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| 基金项目： | 国家自然科学基金资助项目(No.39670305);CMB资助项目(98-681) |
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| 摘 要： | 目的:探讨细菌对大鼠β-防御素-2(rBD-2)基因的表达调控。方法:将大肠杆菌E.coliML-35p和绿脓杆菌ATCC27853大鼠气管内接种,于24h后提取肺组织RNA,用逆转录-聚合酶链反应(RT-PCR)法和Northernblot检测大鼠β-防御素-2基因的表达变化。结果:气管内接种大肠杆菌24h后使大鼠肺组织β-防御素-2mRNA的表达受到显著抑制,绿脓杆菌对其基因的表达却没有抑制作用。结论:大肠杆菌感染可负相调节大鼠肺组织rBD-2mRNA的表达。
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| 关 键 词： | 大鼠 肺 基因表达 肠杆菌科 |
| 文章编号： | 1000-4718(2002)01-0083-03 |
| 收稿时间： | 2000-08-04 |
| 修稿时间： | 2000-08-04 |
Down-regulation of β-defensin-2 gene expression in E.coli-infected rat lung tissue |
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| CHEN Xin-nian,HUANG Ning,WU Qi,WANG Bo-yao. Down-regulation of β-defensin-2 gene expression in E.coli-infected rat lung tissue[J]. Chinese Journal of Pathophysiology, 2002, 18(1): 83-85 |
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| Authors: | CHEN Xin-nian HUANG Ning WU Qi WANG Bo-yao |
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| Affiliation: | Research Unit of Infection and Immunity, Department of Pathophysiology, West China Medical Center, Sichuan University, Chengdu 610041, China |
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| Abstract: | AIM:To study the regulation of rat β-defensin-2 gene expression by bacteria.METHODS:E.coli ML-35 p and pseudomonas aeruginosa were injected into rat trachea. Total RNA was isolated from lung tissue after 24 h inoculation. RT-PCR and Northern blot were performed to detect β-defensin-2 (rBD-2) mRNA expression.RESULTS:The expression of rBD-2 gene was inhibited in the lung tissue by E.coli infection, but not by pseudomonas aeruginosa.CONCLUSION:These results indicated that E.coli infection could down-regulate rBD-2 gene expression in the rat lung tissues. |
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| Keywords: | Rats Lung Gene expression Enterobacteriaceae |
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