基因芯片分析补阳还五汤对局灶性脑缺血再灌注大鼠的保护作用机制

目的 :用基因芯片分析补阳还五汤 (BYHWT)对脑缺血再灌注损伤大鼠的基因表达影响 ,探讨其保护作用机制。方法 :以博星公司定制的包含 512条大鼠的cDNA基因表达谱芯片 ,研究脑缺血 2h再灌注 2 4h的大鼠脑组织基因表达谱 ,并观察补阳还五汤对大鼠脑缺血再灌注损伤的基因表达影响。结果 :脑缺血 2h再灌注 2 4h的大鼠脑组织共筛选出差异表达基因 149条 ,其中 69条基因表达上调 ,80条表达下调 ;脑缺血同时灌胃BYHWT大鼠脑组织共筛选出差异表达基因 31条 ,其中 2 5条基因表达上调 ,6条表达下调。结论 :补阳还五汤对大鼠局灶性脑缺血再灌注损伤后的许多基因表达变化都有调节作用 ,是其保护脑缺血再灌注损伤的机制。

DNA microarray analysis of protective mechanism of Buyang Huanwu decoction on focal cerebral ischemia/reperfusion in rats

Objective: To analyse the effects of Buyang Huanwu decoction(BYHWT) on differentially expressed genes during cerebral ischemia/reperfusion in rats with DNA microarray. Method: cDNA microarray chips containing 512 cDNAs were made by Biostar Genechip Inc. Sprague-Dawley rats were subjected to 2h of middle cerebral artery occlusion (MCAO) with an filament. Saline or BYHWT was given po after onset of cerebral ischemia and brains were removed after 24 h of recirculation for mRNAs isolation. A differential measurment of mRNAs from post-ischemic and BYHWT treated animals was performed with microarray. Result: Up-and down-regulated genes were 69 and 80 in ischemic group. Up-and down-regulated genes were 25 and 6 in BYHWT treated group. Conclusion: BYHWT regulates the differential expression genes after focal brain ischemia/reperfusion in rats,due to its mechanism of protecting cerebral ischemia/reperfusion injury.