| 不耐热肠毒素和耐热肠毒素基因的融合 |
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| 引用本文： | 张兆山,李淑琴,董自正,黄翠芬.不耐热肠毒素和耐热肠毒素基因的融合[J].中华微生物学和免疫学杂志,1994(4). |
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| 作者姓名： | 张兆山 李淑琴 董自正 黄翠芬 |
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| 作者单位： | 军事医学科学院生物工程研究所 |
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| 基金项目： | 国家“八六三”计划资助 |
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| 摘 要： | 用重组基因技术将产肠毒素大肠杆菌（ＥＴＥＣ）的耐热肠毒素（ＳＴａ）基因与不耐热肠毒素Ｂ亚单位（ＬＴ－Ｂ）基因融合。表达的ＬＴＢ／ＳＴａ融合蛋白，既有ＬＴ－Ｂ的抗原性又有ＳＴａ的抗原性。由于基因融合的方式不同，对ＳＴａ的抗原性影响很大，但对ＬＴ－Ｂ的抗原性没有影响。ＬＴ－Ｂ／ＳＴａ融合多肽保留了ＥＴＥｃ肠毒素结合ＧＭ－１神经节甙酯的能力，却仍具有ＳＴａ的生物毒性。
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| 关 键 词： | 产肠毒素大肠杆菌 肠毒素 基因融合 |
FUSION OF GENES ENCODING ESCHERICHIACOLI HEAT-LABILE AND HEAT-STABLE
ENTEROTOXINS |
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| Zhang Zhao-shan,Li Shu-qin,Dong Zi-zheng,et al.FUSION OF GENES ENCODING ESCHERICHIACOLI HEAT-LABILE AND HEAT-STABLE
ENTEROTOXINS[J].Chinese Journal of Microbiology and Immunology,1994(4). |
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| Authors: | Zhang Zhao-shan Li Shu-qin Dong Zi-zheng |
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| Abstract: | he fusion
genes,comprising the genes that code for the B subunit of the heat-labile
enterotoxin(LT-B)andthe heat-stable enterotoxin(STa)of enterotoxigenic E scherichia coli(ETEC).
have been constructed by recombi-nant genetic techniques.The LT-B/STa fusion peptides were
not only of ST antigenicity but also of LT-B anti-genicity. For the different constructions,the
expression levels of ST antigenicity differred greatly , but LT anti-genicity were similar.The
fusion proteins retained the ability to bind to GM-1 ganglioside and native toxicity ofSTa. |
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| Keywords: | Enterotoxigenic Escherichia coli(ETEC) Enterotoxin Gene fusion |
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