当归地上部分中绿原酸和金丝桃苷的含量测定

目的建立当归地上部分中绿原酸与金丝桃苷的含量测定方法。方法采用高效液相色谱法,色谱柱为GRACE C18（250mm×4.6mm,5μm）;流动相为乙腈（A）-0.5%甲酸水溶液（B）梯度洗脱;流速1.0ml/min;检测波长为325nm（绿原酸）和355nm（金丝桃苷）。结果绿原酸和金丝桃苷的线性范围分别为0.29～5.8μg/μL（R2=0.9996）,0.1～2.0μg/μL（R2=0.9999）;平均加样回收率分别为为97.34%（RSD=4.77%）,101.70%（RSD=3.07%）。结论该方法准确可靠、简便可行,可用于当归地上部分药材的质量控制。

Determination of chlorogenic acid and hyperoside in the aerial part of angelica sinensis by HPLC

Objective To establish a HPLC method for the determination of chlorogenic acid and hyperoside in the aerial part of angelica sinensis. Methods The chromatographic separation was achieved on a GRACE C18 （ 250 mm × 4. 6 mm,5 μm） column at a variable wavelength from 325 nm （ Chlorogenic acid） to 355 nm （ hyperoside） using a gradient mobile phase consisted of a mixture of acetonitrile （ A） and 0. 5% formic acid （ B） at a flow rate of 1. 0 ml / min. Results Linearity relationships of chlorogenic acid and hyperoside were 0. 29 ～ 5. 8 μg / μl （ R 2 = 0. 9996 ） ,0. 1 ～ 2. 0 μg / μl （ R 2 = 0. 9999 ） ,respectively; the average recoveries were 97. 34% （ RSD = 4. 77% ） ,101. 70% （ RSD = 3. 07% ） . Conclusion The results showed that the method is accurate,simple,repeatable and it is suitable for the quality control of the aerial parts of Angelica sinensis.