骨髓增生异常综合征患者CD4^＋ CD25^＋调节性T细胞、调控基因FOXP3的表达及意义

目的探讨骨髓增生异常综合征（MDS）患者CD4^＋ CD25^＋调节性T细胞（CD4^＋ CD25^＋ Treg细胞）和调控基因FOXP3的表达及意义。方法分离22例MDS患者MDS组,根据MDS分型标准分为：难治性贫血组（RA组）6例、难治性血细胞减少伴有多系发育异常组（RCMD组）11例、难治性贫血伴原始细胞增多组（RAEB组）5例]和8例正常健康者骨髓单个核细胞,采用免疫荧光标记和流式细胞仪检测CD4^＋ CD25^＋ Treg细胞占CD4^＋ T细胞比例;提取骨髓单个核细胞RNA,采用RT-PCR检测FOXP3基因的表达水平。结果MDS组CD4^＋ CD25^＋ Treg细胞比例、FOXP3基因表达水平与正常对照组比较,差异均无统计学意义（均P〉0.05）;RA组、RAEB组CD4^＋ CD25^＋ Treg细胞比例、FOXP3基因表达水平与正常对照组比较,差异均有统计学意义（均P〈0.01）;RCMD组CD4^＋ CD25^＋ Treg细胞比例、FOXP3基因表达水平与正常对照组比较,差异均无统计学意义（均P〉0.05）;RCMD组、RAEB组CD4^＋ CD25^＋ Treg细胞比例、FOXP3基因表达水平与RA组比较,差异均有统计学意义（均P〈0.01）;RA组、RCMD组CD4^＋ CD25^＋ Treg比例、FOXP3基因表达水平与RAEB组比较,差异均有统计学意义（均P〈0.01）。结论MDS患者CD4^＋ CD25^＋ Treg占CD4^＋ T细胞比例及FOXP3基因表达水平随预后危险级别的升高而升高,提示免疫异常是MDS疾病的发生、发展的一个促进因素。

Expression and Significance of CD4+ CD25+ Treg, FOXP3 Gene in the Myelodysplastic Syndrome

Objective To explore the expression levels and the clinic significance of CD4+CD25+ regulatory T cell (CD4+ CD25+ Treg cell) and regulator and control gene FOXP3 in the myelodysplastic syndrome (MDS) patients.Methods The expression levels of CD4+ CD25+ Treg was detected by means of immunofluorescence and flow cytometry in 24 patients with MDS MDS group:divide by the standard of MDS grouping:refractory anemia group (RA group) 6 examples,refractory cytopenia with multilineage dysplasia group (RCMD group) 11 ...