The role of hepatocyte growth factor in growth plate cartilage |
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| Authors: | Akitoshi Jikko Kazuo Yamashita Masahiro Iwamoto Hiroko Hiranuma Toshiaki Takebayashi Takashi Maeda Mamoru Sakuda Kunio Matsumoto Toshikazu Nakamura Motomi Enomoto-Iwamoto Makoto J Tabata Hajime Fuchihata Kojiro Kurisu |
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| Institution: | (1) Department of Oral Anatomy and Developmental Biology, Osaka University Faculty of Dentistry, 1-8 Yamadaoka, Suita 565-0871, Japan, JP;(2) Department of Radiology, Osaka University Faculty of Dentistry, 1-8 Yamadaoka, Suita 565-0871, Japan, JP;(3) Department of Orthodontics, Osaka University Faculty of Dentistry, 1-8 Yamadaoka, Suita 565-0871, Japan, JP;(4) Department of Biochemistry, Osaka University Faculty of Dentistry, 1-8 Yamadaoka, Suita 565-0871, Japan, JP;(5) Department of Oral Surgery, Okayama University Dental School, Okayama 700, Japan, JP;(6) Division of Biochemistry, Biomedical Research Center, Osaka University Medical School, Suita 565-0871, Japan, JP |
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| Abstract: | To investigate the physiological role of hepatocyte growth factor (HGF) in endochondral bone formation, we examined the expression
of HGF and its receptor c-met and the effects of HGF on growth plate chondrocytes. HGF was highly expressed in the prehypertrophic
zone and hypertrophic zone in rat costal growth plate cartilage. The expression of HGF increased in rabbit chondrocytes as
they matured in culture. Conversely, c-met expression was down regulated along maturation of growth plate chondrocytes. HGF
had weak stimulatory effects on DNA and proteoglycan synthesis of growth plate chondrocytes. However, HGF strongly inhibited
expression of terminal differentiation-related phenotypes, such as type X collagen and alkaline phosphatase (APase) synthesis
and cartilage matrix mineralization. When HGF was removed from the cultures, cells quickly expressed type X collagen and APase.
Once chondrocytes differentiated to mature chondrocytes, HGF did not inhibit further differentiation of these cells. These
results suggested that HGF is a negative regulator of terminal differentiation of growth plate chondrocytes..
Received: Feb. 12, 1998 / Accepted: March 12, 1998 |
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| Keywords: | : HGF growth plate chondrocyte differentiation |
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