红曲提取物对体外培养成骨细胞的作用研究

目的：研究红曲不同提取物对体外培养大鼠成骨细胞（ROB）增殖、分化的影响。方法：采用新生大鼠颅骨分离培养ROB,MTT法测定细胞增殖,碱性磷酸酶（ALP）试剂盒法测定ALP活性,观察红曲提取物对ROB增殖、分化的作用,同时考察了MTT法和ALP试剂盒法中细胞数和吸收值之间的线性关系。结果：10^-2和10^-3mg/ml的水提物能刺激ROB的增殖（P〈0.05）,10^-3mg/ml的水提物还能提高ROB的ALP活性（P〈0.05）;10^-2和10^-3mg/ml的95%乙醇提取物对ROB的增殖与分化均有明显的促进作用（P〈0.05或P〈0.01）。结论：红曲水提物和95%乙醇提取物均能促进ROB骨形成过程中增殖、分化,95%乙醇提取物作用效果显著。

Study of Fermented Red Yeast Rice extract on osteoblast in vitro

Objective: Study on the effect of the extracts of fermented red yeast rice (RYR) on cell proliferation and differentiation in cultured rat osteoblast. Method: The effects of the extracts on proliferation and differentiation were studied respectively by using MTT and ALP assays in newborn rat calvarial (ROB) cell. The relationships between cell number and the absorbance of MTT and ALP assays were examined. Result: The aqueous extract concentration of 10-2 and 10-3 mg/ml showed the effects on proliferation in cell (P<0.05), and 10-3 mg/ml of aqueous extract showed the effect on differentiation in cell (P<0.05); the 95% alcohol extract concentration of 10-2 and 10-3mg/ml both showed significant effects on proliferation and differentiation in cell (P<0.05 or P<0.01). Conclusion: Both of the aqueous extract and the 95% alcohol extract can promote proliferation and differentiation of ROB cell in vitro, and the 95% alcohol extract showed a significant effect.