Apelin-13促血管平滑肌细胞增殖作用研究

目的研究G蛋白偶联受体APJ的内源性配体Ape-lin-13对血管平滑肌细胞(vascular smooth muscle cells,VSMC)生长增殖影响及其与细胞周期蛋白D1(cyclin D1)的关系,发现Apelin-APJ新功能。方法培养大鼠胸主动脉血管平滑肌细胞,噻唑蓝比色法(MTT)观察VSMC增殖;流式细胞仪分析细胞周期;Western blot检测cyclinD1的表达。结果Apelin-13能促进血管平滑肌细胞增殖,降低G0/G1期细胞百分比,升高S+G2+M期细胞百分比,并可剂量依赖性刺激细胞cyclin D1的表达增加。结论Apelin-13能通过推动细胞周期由G0/G1期进入S期而促进血管平滑肌细胞增殖,其作用机制与促进cyclin D1表达增高有关。

The study of vascular smooth muscle cells proliferation stimulated by Apelin-13

Aim To observe the effect of G protein-coupled receptor APJ endogenous ligand Apelin-13 on the proliferation of vascular smooth muscle cells (VSMC),and investigate the role of cyclin D1 in this effect. Methods VSMC were prepared from thoracic aortas of male Sprague-Dawley rats by the explant method. The proliferation and cell cycle were measured by MTT assay and Flow Cytometry (FCM),respectively. Expression of cyclin D1 were determined by Western blot. Results Apelin-13 can stimulated VSMC proliferation in a concentration-dependent manner. It decreased the proportion of G0/G1 phase but increased S phase.Furthermore, Apelin-13 promoted the expression of cyclin D1. Conclusion Apelin-13 stimulated the proliferation of VSMC through G0/G1 phase into S phase,and the effect maybe involved in the increased expression of cyclin D1.