应用PCR-SSCP法检测乳腺癌患者血中p53基因突变

[目的]探讨乳腺癌患者外周血p53基因突变的临床意义.[方法]应用聚合酶链反应-单链构象多态性分析（PCR-SSCP）的方法检测35例乳腺癌患者和20例良性肿瘤患者外周血中p53基因外显子5～8的突变情况.[结果]乳腺癌患者p53基因突变的检出率为40.0%（14/35）,显著高于对照组良性肿瘤患者的5.0%（1/20）（P＜0.05）.[结论]p53基因突变在乳腺癌外周血中有较高的检出率,是乳腺癌发生的早期信号.

Plasma p53 gene mutation in primary breast cancer patients

[Objective]To investigate the clinical significance about p53 gene mutation in blood of the patients with breast cancer.[Method]Use of single strand conformation polymorphism analysis of polymerase chain reaction products(PCR-SSCP) to inspect p53 gene mutation from 35 blood samples of the patients with breast cancer and 20 blood samples of the patients with breast benign tumor.[Result]The mutation rate of the patients with breast cancer were 40.0%(14/35),and the mutation rate of the patients with breast benign tumor were only 5.0%(1/20).The difference was significant(P<0.05).[Conclusion]Blood p53 gene mutation from the patients with breast cancer have higher detection rate,which is the early period signal of breast cancer.