| 液氮冻存人T淋巴细胞用于HLA 血清定型试验:方法的改进 |
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| 引用本文： | 李曙光,陈继嵩,孙科柱,易有年. 液氮冻存人T淋巴细胞用于HLA 血清定型试验:方法的改进[J]. 中南大学学报(医学版), 1985, 0(1) |
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| 作者姓名： | 李曙光 陈继嵩 孙科柱 易有年 |
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| 作者单位： | 湖南医学院免疫学研究室(李曙光，陈继嵩，孙科柱)，湖南医学院免疫学研究室(易有年) |
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| 摘 要： | 报告我室参照Terasaki 实验室用液氮冻存人外周血T淋巴细胞方法的改进及其效果。冻存11—14个月后仍可进行HLA—A、B、C血清定型试验。
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| 关 键 词： | T淋巴细胞 冷冻保存 组织相容性试验 方法学 |
CRYOPRESERVED T LYMPHOCYTES IN LIQUID NITROGEN FOR HLA TYPING:METHODOLOGICAL IMPROVEMENT |
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| Li Shuguang Cheng Chisong Sun Kezhu Yi YounianImmunology Research Laboratory,Hunan Medical College. CRYOPRESERVED T LYMPHOCYTES IN LIQUID NITROGEN FOR HLA TYPING:METHODOLOGICAL IMPROVEMENT[J]. Journal of Central South University. Medical sciences, 1985, 0(1) |
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| Authors: | Li Shuguang Cheng Chisong Sun Kezhu Yi YounianImmunology Research Laboratory Hunan Medical College |
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| Abstract: | Procedures in cryopreservation of human peripheral T lymphocytes according to Prof. Terasaki's Laboratory was modified. After 11-14 months of cryopreservation, the viability rate of most samples collected from 26 blood donors and 9 patients with nasopharyngeal carcinoma was above 90% ,and 8 samples used in HLA-A,B,C sera screening test have provided reproducible result. |
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| Keywords: | T-lymphocytes Cryopreservation Histocompatability testing Method |
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