| EXPRESSION OF IL-3 cDNA IN MURINE BONE MARROW CELLS RESULTS IN AUTONOMOUS GROWTH |
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| 引用本文： | 王随照,宋增璇. EXPRESSION OF IL-3 cDNA IN MURINE BONE MARROW CELLS RESULTS IN AUTONOMOUS GROWTH[J]. 中国癌症研究, 1993, 5(1): 28-34. DOI: 10.1007/BF02997490 |
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| 作者姓名： | 王随照 宋增璇 |
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| 作者单位： | Department of Hematology，the first Affiliated Hospital，Henm Medical University，Zhengzhou 450052，Institute of Hematology，Chinese Academy of Medical Sciaces，Tianjm 300020 |
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| 摘 要： | Infection of murine bone marrow cells with retrovirus, containing the interleukin 3(IL-3) coding sequence and the newmyecine resistant gene, resulted In the ggeneration of factor-independent, G418 resistant, and Immortalized cell line E001. The established cell line has unique cytochemistry character, two marker chromosomes, and the Robertsonian translocation of chromosome 10th. The cell needs a stromal-dependent Mage before Us autonomous growth. The E001 cells secrete IL-3 which supports the growth of factor-dependent cell line 32D. The E001 cell growth is not influenced by its own conditioned medium and the cell density. These data Indicate that the autocrine stimulating mechanism may be one of the promary events In the cell transformation, white the expression of IL-3 and/or the retrovirus Insertion may respond to the phenotype of me E001 cells. The stromal layer may also be necessary In the cell transforming process.
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Expression of IL-3 cDNA in murine bone marrow cells results in autonomous growth |
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| Suizhao Wang,Zengxuan Song. Expression of IL-3 cDNA in murine bone marrow cells results in autonomous growth[J]. Chinese Journal of Cancer Research, 1993, 5(1): 28-34. DOI: 10.1007/BF02997490 |
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| Authors: | Suizhao Wang Zengxuan Song |
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| Affiliation: | Wang Suizhao Song ZengxuanDepartment of Hematology,the first Affiliated Hospital,Henm Medical University,Zhengzhou 450052,Institute of Hematology,Chinese Academy of Medical Sciaces,Tianjm 300020 |
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| Abstract: | Infection of murine bone marrow cells with retrovirus, containing the interleukin 3(IL-3) coding sequence and the newmyecine resistant gene, resulted In the ggeneration of factor-independent, G418 resistant, and Immortalized cell line E001. The established cell line has unique cytochemistry character, two marker chromosomes, and the Robertsonian translocation of chromosome 10th. The cell needs a stromal-dependent Mage before Us autonomous growth. The E001 cells secrete IL-3 which supports the growth of factor-dependent cell line 32D. The E001 cell growth is not influenced by its own conditioned medium and the cell density. These data Indicate that the autocrine stimulating mechanism may be one of the promary events In the cell transformation, white the expression of IL-3 and/or the retrovirus Insertion may respond to the phenotype of me E001 cells. The stromal layer may also be necessary In the cell transforming process. |
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| Keywords: | Interleukin 3 Infection Gene expression Transtormation. |
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