晶体上皮细胞在体外的分化规律及血清和眼组织对其增殖的影响

目的从细胞培养水平探讨后囊混浊形成机理和术后血－房水屏障破坏、晶体皮质残留等对其的影响。方法用考马斯亮蓝（ＣｏｏｍａｓｓｉｅＢＢ）染色、倒置显微镜和电镜观察培养的牛晶体上皮细胞（ｂｏｖｉｎｅｌｅｎｓｅｐｉｔｈｅｌｉａｌｃｅｌｓ，ＢＬＥＣ）的增殖和分化规律，用Ｇｉｅｍｓａ染色比色法观察胎牛血清、房水、晶体皮质等对ＢＬＥＣ增殖的影响。结果在体外培养条件下ＢＬＥＣ可在第１～７代内分化为晶体纤维细胞，表现为细胞体积增大，形状渐趋长条形和梭形，细胞骨架逐渐增多；胎牛血清呈浓度依赖性促进ＢＬＥＣ增殖（Ｐ＜０．０５）；高浓度房水（占培养液１／３）抑制ＢＬＥＣ增殖（Ｐ＜０．０１），晶体皮质、晶体核、玻璃体均促进ＢＬＥＣ增殖（Ｐ＜０．０１）。结论晶体上皮细胞的分化在后囊混浊形成中起重要作用；术后血－房水屏障破坏、晶体皮质残留和玻璃体脱出可通过刺激晶体上皮细胞增殖促进后囊混浊形成。

Differentiation of lens epithelial cell and effects of serum and ocular tissue on its proliferaton in vitroi

Objective To study the pathogenesis of lens posterior capsular opacification (PCO) and the effects of the breakdown of blood aqueous barrier and remain of lens cortex on PCO in cell culture level.Methods The differentiation of cultured bovine lens epithelial cells (BLEC) was observed by Coomassie BB staining,light and electon microscope. The effects of fetal bovine serum,aqueous humor and lens cortex,etc.on the proliferation of BLEC were tested by Giemsa staining colorimety.Results BLEC in vitro could gradually differentiate into lens fiber cells or lens fibers in 1 7 passages. The characteristics of differentiation were that the shape of cells changed from golgyon into fusifrom and fibrifrom, the bulk enlarged,and cytoskeleton gradually incrdased..Fetal bovine serum promoted the proliferation of BLEC depending on its concentration ( P <0 05).High concentration of aqueous humor inhibited the proliferation ( P <0 01). Supernatant and suspension of lens cortex, supernatant of lens nucleus,vitreous humor could promote the proliferation ( P <0 01).Conclusion The differentiation of lens epithelial cells plays important roles in PCO,the breakdown of blood aqueous barrier,the reman of lens cortex and the prolapse of vitrous may promote PCO by stimulating the proliferation of lens epithelial cells.