人眼小梁细胞体外表达表皮生长因子mRNA和表皮生长因子受体

目的 了解培养的小梁细胞分泌表皮生长因子（ｅｐｉｄｅｒｍａｌ ｇｒｏｗｔｈ ｆａｃｔｏｒ,ＥＧＦ）及细胞膜上表皮生长因子受体（ｅｐｉｄｅｒｍａｌ ｇｒｏｗｔｈ ｆａｃｔｏｒ ｒｅｃｅｐｔｏｒ,ＥＧＦＲ）的情况。方法 进行人眼小梁细胞体外培养。用ＥＧＦ ｃＤＡＮ探针,α＾－３２ｐ同位素标记及斑点杂交放射自显影法,检测小梁细胞分泌ＥＧＦｍＲＮＡ的情况。结果 人眼小梁细胞体外培养成功。免疫组化染色显示小

The expression of EGF mRNA and EGF receptors in human trabecular meshwork cells in vitro

OBJECTIVE: To demonstrate that cultured trabecular meshwork cells can secrete epidermal growth factor (EGF) and there are EGF receptors (EGFRs) on the cells. METHODS: Human trabecular meshwork cells were cultured in vitro and passaged 3-5 times. Immunohistochemical stain was used to detect EGFRs on the trabecular meshwork cell membrane. EGF cDNA probe, alpha-32P isotope labeling and dot blot hybridization autoradiographic method were used to detect EGF mRNA in the cells. RESULTS: Cultured human trabecular meshwork cells were obtained. EGFR immunostain was positive, and the reaction of brown color was at the surface of the cells. Dot blot hybridization autoradiography showed that trabecular cells can express EGF mRNA. CONCLUSIONS: Trabecular meshwork cells can secrete EGF. There are EGFRs on the membrane of trabecular meshwork cells. It is suggested that probably up-regulating the receptors or promoting the cells to secrete growth factors have potential significance in the regeneration of trabecular cells and recovery of the cell functions in open angle glaucoma.