人类白细胞抗原—DR4基因亚型与Vogt—Koyanagi—Harada综合征相…

研究人类白细胞抗原－ＤＲ４基因亚型与Ｖｏｇｔ－Ｋｏｙａｗｎａｇｉ－Ｈａｒａｄａ综合征的关系，从免疫遗传学角度阐明ＶＫＨ的易感性及抵抗性。方法 采用聚合酶链反应－限制性片段长度多态性分析方法，检测４例ＶＨＫ综合征患者和１０６例健康对照者的ＥＲ４－ＤＲＢ１基因亚型。结果 ５４例ＶＫＨ综合征患者中，３８例扩增出２６３ｂｐ的特异性ＤＲ－４－ＤＲＢ１基因片段，阳性检出率为７０％，对照组仅为１９％，相对危险度

The association of HLA-DR4 gene subtypes with Vogt-Koyanagi-Harada syndrome]

OBJECTIVE: To investigate the association of HLA-DR4 subtypes with Vogt-Koyanagi-Harada (VKH) syndrome and to clarify immune genetic mechanism underlying the susceptibility/resistance to VKH syndrome. METHODS: HLA-DR4 alleles of 54 patients with VKH and 106 healthy controls were amplified and subtyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). RESULTS: A selective amplification of the DR4 alleles of 263 bp was higher in patients with VKH than in the controls. The frequency of DR4-DRB1 alleles was 70% (38/54) in VKH group and was 19% (20/106) in the control group, relative rish (RR) = 10.21, P < 0.005. The genotyping of DR4 alleles showed that DRB1 * 0405 was more frequent in VKH group (35/38, 92%) than in the control group [6/20, 30%, RR = 27.2, P = 0.0,000,017, corrected pvalue (Pc) = 0.0,000,103]. CONCLUSIONS: It is suggested that DRB1 * 0405 be a susceptible subtype to VKH and the specific amino acid residue Ser (serine) at position 57 play an important role in the development of VKH syndrome. The molecular biological technique we used may be useful in studying the immunogenetic mechanism of VKH.