沙门菌毒力岛1缺失山夫登堡沙门菌的特性

目的 研究沙门菌毒力岛1( SPI-1)缺失山夫登堡沙门菌的特性。方法 于2006年从上海10例腹泻患者粪便中分离得到10株山夫登堡沙门菌,运用脉冲场凝胶电泳(PFGE)、PCR、毒力基因测序及细胞侵袭试验对其进行分子分型、侵袭基因及细胞侵袭能力检测,并与2002年深圳分离株( C02013)进行比较。结果 10...

Characteristics of Salmonella enterica serovar Senftenberg lacking Salmonella pathogenicity island 1

ObjectiveTo study the characteristics of the strains of Salmonella enterica ( S. enterica) serovar Senfienberg lacking Salmonella pathogenicity island 1 ( SPI-1 ). Methods A total of 10 strains of S. enterica serovar Senfienberg were isolated from 10 cases of diarrhea patients. Pulsed field gel electrophoresis (PFGE), PCR, sequencing techniques and cell invasion test were adapted to study the molecular types and invasiveness of the genes and cells; and made a comparison between the 10 strains and the strains ( C02013 ) isolated in Shenzhen in 2002. Results The 10 Senftenberg isolated ( S09007 -S09012, S09014 - S09017 ) in Shanghai showed three PFGE patterns, which were significantly different from the strains isolated in Shenzhen. PCR-amplified results indicated the invasion gene ( invA ), secreted effector protein gene (sipA) and gene fragments as fhlA-hilA ,hilA-spaP and spaP-invH in the 10 strains of SPI-1 were all negative. The sequencing results revealed that the 10 strains isolated in Shanghai lacked most parts of SPI-1 genes,as fragments from orgA to invH and parts of orgA gene itself; however,compared with strains isolated in Shenzhen, the sprB-orgC gene existed. The missing parts of genes were replaced by a simple insertion sequence (IS) of 1000 bp in the strains isolated both in Shenzhen in 2002 and in Shanghai in 2006. The invasiveness rates of the 10 strains ( S09007 - S09012, S09014 - S09017 ) towards Hela cells were (0.0053 ±0.0024)％, (0. 0046 ±0.0006)％ ,(0.0047 ±0.0003)％ ,(0.0064 ±0.0012)％ ,(0.0065 ±0. 0011 )％, (0. 0070 ±0. 0020)％, (0. 0115 ± 0. 0030)％, (0. 0099 ± 0. 0039)％, (0. 0180 ± 0. 0135)％and (0. 0031 ± 0. 0012)％, respectively; which were all significantly lower than the rate of invA-positive control strain STM1344 ((5.0800 ± 0.6333)％); lower or close to the rate of invA-lacked artificial-mutated strain STMinvA - ( (0. 0193 ± 0. 0045) ％ ). Conclusion SPI-1 genes are not essential for the diarrhea caused by S. enterica serovar Senftenberg.