2009—2010年山东省食品中单核细胞增生李斯特菌的耐药性和分子分型研究

目的 了解山东省单核细胞增生李斯特菌(单增李斯特菌)耐药及分子分型特点,并探讨耐药表型与PFGE基因组型之间的关系.方法 从2009-2010年山东省6个城市的生畜禽肉、熟肉制品、水产品等食品中采集80株单增李斯特菌,采用微量肉汤稀释法测定其抗生素敏感性,采用PFGE进行分子分型,并对耐药谱与PFGE型别关系进行分析.结果 16.25％(13/80)的菌株存在耐药,其中,业胺培南的耐药率最高(12.50％,10/80),其次是四环素和强力霉素(3.75％,3/80;2.50％,2/80).耐药分型可将80株菌分为9个型别.PFGE将80株菌分为34个带型,以17型和29型为主.A型耐药谱分布范围较广,出现在79.41％ (27/34)的PFGE型别中.结论 2009-2010年山东省单增李斯特菌存在较为严重的耐药现象,单增李斯特菌的PFGE分型与其药敏分型无对应关系.

Antibiotic resistance and molecular typing of Listeria monocytogenes from foods in Shandong province from 2009 to 2010

Objective To know the antibiotic resistance and molecular characteristics of Listeria monocytogenes in Shandong province and to study the relationship between antibiotic resistance phenotypes and genome types.Methods From 2009 to 2010,a total of 80 Listeria monocytogenes isolates were collected from raw meat,cooked meat,aquatic products and other foods in 6 cities of Shandong province.The antibiotic susceptibility was measured by broth microdilution method,PFGE was performed for molecular typing and the relationship between antimicrobial resistance and PFGE patterns was analyzed.Results 16.25％ (13/80) of the isolates were drug-resistant.Imipenem resistance was the most prevalent (12.50％,10/80),followed by tetracycline and doxycycline (3.75％,3/80 and 2.50％,2/80).A total of the 80 isolates were subtyped into 9 antibiotic resistance patterns and 34 PFGE types which were largely dominated by the type 17 and 29.Antibiotic resistance pattern A corresponded to 79.41％ ( 27/34 ) of PFGE types.Conclusion The antibiotic resistance of Listeria monocytogenes in Shandong province is serious from 2009 to 2010 and there is no correlation between PFGE types and antibiotic resistance patterns.