Analysis of DNA methylation in human BK virus |
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| Authors: | Chi-Fang Chang Meilin Wang Chiung-Yao Fang Pei-Lain Chen Shu-Fen Wu Michael W Y Chan Deching Chang |
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| Institution: | (1) Institute of Molecular Biology, National Chung Cheng University, 168, University Rd., Min-Hsiung, Chia-Yi, 621, Taiwan;(2) Department of Chemistry and Biochemistry, National Chung Cheng University, Chia-Yi, Taiwan;(3) Department of Microbiology and Immunology, Chung Shan Medical University, Taichung, Taiwan;(4) Department of Urology, Chia-Yi Christian Hospital, Chia-Yi, Taiwan;(5) Department of Medical Laboratory Science and Biotechnology, Central Taiwan University of Science and Technology, Taichung, Taiwan; |
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| Abstract: | Human BK virus may cause nephropathy due to viral replication in patients who have undergone renal transplantation. However,
the mechanism regulating replication of BKV is still not clear. Previous studies have suggested that epigenetic modifications
may play a crucial role in virus replication. In this study, the DNA methylation profiles of five CpG sites located within
the promoter/enhancer regions and nine CpG sites located within the early and late coding regions of the replicating BKV genome
were investigated. BKV genomic DNA from mature virions and from the early and late phases of replicating BKV were examined
for DNA methylation by bisulfite sequencing that covered 14 CpG sites. Our results showed that none of the examined BKV DNA
from the various different stages of replication was methylated. This is the first report to analyze the methylation of BKV
genomic DNA during viral replication. The results seem to indicate that methylation is not involved in regulation of BKV replication. |
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