慢性高原病患者骨髓造血细胞凋亡及caspase-8和caspase-9表达研究

目的 探讨慢性高原病( CMS)患者骨髓造血细胞凋亡指数及caspase-8和caspase-9mRNA表达变化的意义.方法 选取18例CMS患者,采用末端脱氧核苷酸转移酶介导的dUTP缺口标记(TUNEL)技术定量研究骨髓单个核细胞(BMMNC)凋亡指数,同时采用RT-PCR半定量方法检测BMMNC caspase-8和caspase-9 mRNA的表达.以16例骨科单纯骨折患者为对照.结果 ①CMS患者BMMNC凋亡指数[(8.51±3.35)％]明显低于对照组[(16.00±4.28)％](P＜0.01).②CMS患者caspase-8和caspase-9 mRNA的相对表达水平分别为0.28 ±0.07和0.23±0.08,对照组分别为0.45±0.09和0.41±0.09,CMS组均明显低于对照组(P值均＜0.01).③CMS患者caspase-8和caspase-9mRNA表达水平与血红蛋白浓度呈负相关(r值分别为-0.520及-0.610,P值均＜0.05),与凋亡指数均未发现明显相关性(P值均＞0.05),CMS患者凋亡指数与血红蛋白浓度呈负相关(r=-0.890,P＜0.01).结论 CMS患者BMMNC凋亡指数及caspase-8和caspase-9表达水平均降低,提示CMS患者骨髓造血细胞凋亡减少,caspase-8和caspase-9均参与CMS骨髓造血细胞凋亡异常机制.

Studies on apoptosis and caspase-8 and caspase-9 expressions of bone marrow cells in chronic mountain sickness

Objective To observe the expressions of caspase-8 and caspase-9 mRNA,and explore the changes of apoptosis of bone marrow hematopoietic cells in patients with chronic mountain sickness (CMS).Methods Of 18 CMS patients and 16 controls were enrolled in this study.The apoptotic index (AI) of bone marrow mononuclear cells(BMMNC) was measured by TUNEL technique,the levels of caspase-8 and caspase-9 mRNA in BMMNC of CMS patients and controls were determined by RT-PCR.Results (①The AI of BMMNC in patients with CMS ( 8.51 ± 3.35 ) ％ was lower than that in controls (16.00±4.28)％ (P＜0.01) ; ②The values of caspase-8 and caspase-9 mRNA were (0.28 ±0.07) and (0.23 ±0.08) respectively,in CMS patients,which were significantly lower than those of (0.45 ± 0.09) and ( 0.41 ± 0.09 ) respectively,in the controls ( both P ＜ 0.01 ) ; ③Hemoglobin (Hb) value was negatively correlated with levels of caspase-8 and caspase-9 mRNA (r values were -0.52 and -0.61 respectively,both P ＜ 0.05) in CMS patients.There was a negative correlation between AI and Hb( r value was -0.89,P ＜ 0.01 ) in CMS patients.However,the significant relationship was not found between AI and level of caspase8 or caspase-9 mRNA( P ＞ 0.05).Conclusions The results showed a decrease apoptosis of BMMNCs and reduced levels of caspase-8 and caspase-9 mRNA in CMS patients,the latter might be involved in the change of BMMNCs apoptosis.