钛表面载银HA-TCP溶胶凝胶涂层的制备及其抗菌性的研究

目的：用溶胶凝胶法在钛表面制备载银HA-TCP涂层，并观察其抗菌性能。方法：采用溶胶凝胶法将Ag^＋导入HA-TCP溶胶，并在钛表面涂层。用X射线衍射（XRD）和傅立叶变换红外光谱（FTIR）对涂层结构进行表征分析，用X射线能谱（EDS）分析涂层表面元素构成和分布，用扫描电镜（SEM）观察涂层表面形貌。观察涂层对牙龈卟啉单胞菌P.g、具核梭杆菌F.n、伴放线放线杆菌A-a的生长抑制作用，并计算抗菌率。结果：载银HA-TCP溶胶在钛表面涂层均匀，由大量的亚微米及细微的介孔组成。XRD检测出HA、钛、锐钛矿和β-TCP的衍射峰；FTIR分析涂层含有OH^-．PO4^3-及CO3^2-的特征吸收峰。EDS检测发现钙、磷、钛、碳、氧和银在涂层表面的分布。与对照组比较，载银HA-TCP涂层对牙龈卟啉单胞菌P.g、具核梭杆菌F.n、伴放线放线杆菌A.a具有明显的抗菌效果。结论：本试验在钛表面涂覆载银HA-TCP溶胶，使钛植入体获得了具有生物活性及抗菌性的仿生涂层。

The Antibacterial properties of silver-containing hydroxyapatite/TCP coatings produced using a sol gel process

PURPOSE： To introduce the silver-containing hydroxyapatite/TCP coatings using a sol gel process and to investigate the effect of coatings on antibacterial adhesion. METHOD： Using a sol-gel process, HA/TCP coatings doped with AgNO3 were prepared.The coated surfaces were characterized using XRD, FTIR, EDS and SEM. The antibacterial properties were evaluated using P.gingivalis, F.nucleatum and A.actinobacillus. RESULTS： XRD and FTIR analysis of surfaces detected peaks corresponding to HA/TCP coatings. SEM showed the surface of coating was even and covered with a number of micropores. EDS detected calcium, phosphorus, titanium, carbon, oxygen and silver elements on the coatings. In vitro antibacterial study indicated a reduced number of P.gingivalis, Enucleatum and A.actinobacillus on Ag-HA/TCP surfaces when compared with HA/TCP surface. The antibacterial ability was ordered as P.gingivalis〉 F.nucleatum〉A. actinobacillus. CONCLUSIONS： Silver-containing hydroxyapatite/TCP coatings with good physical and chemical structure have the ability to minimize the bacteria adhesion and growth, and it is a promising candidate as an antibacterial and osteogenic coatings for dental implant.