AG490对谷氨酸诱导PC12细胞磷酸化STAT3活性上调的抑制作用

目的研究AG-490拮抗谷氨酸对PC12细胞中P-STAT3活性的上调作用及其抗凋亡意义。方法谷氨酸诱导PC12细胞凋亡。Hochest荧光染色观察PC12细胞形态。流式细胞仪(FCM)分析评价凋亡百分率。Westernblot测定P-STAT3蛋白表达量,MTT检测PC12细胞存活率。结果谷氨酸诱导PC12细胞中STAT3蛋白表达上调(P=0.000),如予Jak/STAT3信号通路阻断剂AG-490预处理,则其表达被下调(P=0.002),细胞凋亡率减低(P=0.000),细胞存活率升高(P=0.000)。结论AG-490拮抗谷氨酸诱导的PC12细胞凋亡,其机制可能与STAT3活化有关。

Inhibitory Effects of AG - 490 on Apoptosis of PC12 Cell Induced by Glutamate

Objective To investigate the rivalry effects of AG-490 with glutamic acid on the activation of P-STAT3 which resists apoptosis of PC12 cells.Methods Glutamic acid was used to induce apoptosis of PC21 cells.Cell morphology was observed by Hochest fluorescent staining.Apoptosis percentage was analyzed by flow cytometry.P-STAT3 protein expression was detected by Western blotting,and survival rate of PC12 cells was detected by MTT.Results Protein expression of STAT3 up-regulated in PC21 cells induced by glutamic acid(P=0.000).AG-490 was used to block Jak/STAT3 signal access,thus the protein expression down-regulated,apoptosis rate of PC12 cell reduced and survival rate improved.Conclusion AG-490 can antagonize the glutamic acid induced apoptosis of PC12 cells,and the underlying mechanism might be concerned with activation of STAT3 protein.