| Elafin在人支气管上皮细胞系16HBE胞质内对黏蛋白5AC生成的影响 |
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| 引用本文： | 魏华生,尤列·皮尔曼,维克多·科罗索夫,周向东.Elafin在人支气管上皮细胞系16HBE胞质内对黏蛋白5AC生成的影响[J].基础医学与临床,2011,31(7):809-813. |
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| 作者姓名： | 魏华生 尤列·皮尔曼 维克多·科罗索夫 周向东 |
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| 作者单位： | 1. 重庆医科大学,附属第二医院,呼吸内科,重庆,400010
2. 俄罗斯医学科学院,远东呼吸生理与病理中心,布拉戈维申斯克,675000 |
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| 基金项目： | 国家自然科学基金(81070031);国家自然科学基金中俄合作项目(81011120108) |
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| 摘 要： | 目的 探讨Elafin在胞质内对表皮生长因子(EGF)诱导的黏蛋白(MUC)5AC生成的影响及其作用机制.方法 体外培养人支气管上皮细胞系16HBE,将已构建好的pEGFP-N1-Elafin载体通过LipofectamineTM2000转染到16HBE细胞中,以转染空质粒载体的16HBE细胞作为对照,均给予外源性EG...
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| 关 键 词： | Elafin 核因子-κB 小泛素样修饰蛋白-1 类泛素化 黏蛋白5AC |
The effect of Elafin in 16HBE cytoplasm on the mucin5AC production |
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| WEI Hua-sheng,Juliy M.Perelman,Victor P.Kolosov,ZHOU Xiang-dong.The effect of Elafin in 16HBE cytoplasm on the mucin5AC production[J].Basic Medical Sciences and Clinics,2011,31(7):809-813. |
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| Authors: | WEI Hua-sheng Juliy MPerelman Victor PKolosov ZHOU Xiang-dong |
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| Institution: | WEI Hua-sheng1,Juliy M.Perelman2,Victor P.Kolosov2,ZHOU Xiang-dong1(1.Dept.of Respiratory Medicine,the Second Affiliated Hospital,Chongqing University of Medical Science,Chongqing 400010,China,2.Far Eastern Scientific Center of Physiology and Pathology of Respiration,Blagoveschensk 675000,Russia) |
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| Abstract: | Objective To investigate the effect of Elafin in cytoplasm on epidermal growth factor(EGF)-induced mucin(MUC)5AC production and the mechamism.Methods After cultivating 16HBE cells in vitro,the constructed pEGFP-N1-Elafin eukaryotic expression vector was transfected into the 16HBE cells by LipofectamineTM2000,and 16HBE cells transfected the vector without Elafin gene were classified as control group.The cells were then incubated with exogenous EGF.The mucin(MUC)5AC mRNA level was analyzed by RT-PCR,the MUC5A... |
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