| MicroRNA-144调控红系分化 |
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| 引用本文： | 汪晓艳,蒋崇亮,朱勇,王芳,余佳,冯涛.MicroRNA-144调控红系分化[J].基础医学与临床,2011,31(6). |
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| 作者姓名： | 汪晓艳 蒋崇亮 朱勇 王芳 余佳 冯涛 |
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| 作者单位： | 1. 重庆医科大学,基础医学院,分子医学与肿瘤研究中心,重庆,400016
2. 中国医学科学院,基础医学研究所北京协和医学院,基础学院,医学分子生物学国家重点实验室,北京,100005 |
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| 基金项目： | 国家自然科学基金(81071770) |
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| 摘 要： | 目的研究microRNA-144(miR-144)通过调节视网膜母细胞瘤蛋白(RB)对红系分化的影响。方法用氯化高铁血红素(hemin)诱导人慢性髓系白血病细胞系K562可实现在体外模拟红系分化过程,使用Real-time PCR检测miR-144表达;利用体外合成的寡核苷酸(mimic-144)转染K562细胞,检测过量表达miR-144对红系分化的影响;结合生物信息学分析、双荧光素酶报告系统和Western blot寻找并确定miR-144的靶基因。结果 miR-144在hemin诱导的K562红系分化过程中表达显著升高(P<0.05),在K562细胞中过表达miR-144可以促进血红蛋白(γ-globin)和红细胞表面标志CD235a的表达和积累;RB为miR-144的靶基因之一;在K562红系分化中,RB呈先略升后降的趋势,以0 h为参照,12 h RB/GAPDH灰度值最低为:0.092±0.007(P<0.05)。结论 miR-144通过负调控RB的表达促进hemin诱导的K562红系分化。
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| 关 键 词： | microRNA-144 红系分化 视网膜母细胞瘤蛋白 |
MicroRNA-144 regulates erythroid differentiation of K562 cells |
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| WANG Xiao-yan,JIANG Chong-liang,ZHU Yong,WANG Fang,YU Jia,FENG Tao.MicroRNA-144 regulates erythroid differentiation of K562 cells[J].Basic Medical Sciences and Clinics,2011,31(6). |
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| Authors: | WANG Xiao-yan JIANG Chong-liang ZHU Yong WANG Fang YU Jia FENG Tao |
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| Institution: | WANG Xiao-yan1,JIANG Chong-liang1,ZHU Yong1,WANG Fang2,YU Jia2,FENG Tao1(1.Molecular Medicine & Cancer Research Center,Institute of Basic Medical Sciences,Chongqing Medical University,Chongqing 400016,2.National Laboratory of Medical Molecular Biology,CAMS & PUMC,Beijing 100005,China) |
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| Abstract: | Objective We aimed to study the influence of miR-144 on human erythroid differentiation via targeting the retinoblastoma protein(RB).Methods K562 cells were treated with hemin to differentiate into mature red cells.Real-time PCR analysis was used to detect the changes of miR-144 expression during erythroid differentiation.K562 cells were transfected with oligonucleotides(mimic-144) and the influence of erythropoiesis was measured.The target gene of miR-144 was identified by using bioinformatics analysis com... |
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| Keywords: | microRNA-144 erythroid differentiation the retinoblastoma protein |
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