| MUC1靶向性载药纳米粒的构建及其体外抗肿瘤效应的评估 |
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| 引用本文： | 于琛琛,胡燕,段金虹,王琛,许海燕,杨先达. MUC1靶向性载药纳米粒的构建及其体外抗肿瘤效应的评估[J]. 基础医学与临床, 2011, 31(7) |
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| 作者姓名： | 于琛琛 胡燕 段金虹 王琛 许海燕 杨先达 |
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| 作者单位： | 1. 中国医学科学院,基础医学研究所,北京,100005
2. 国家纳米科学中心,北京,100190 |
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| 基金项目： | 国家重大科学计划(2011CB911003,2011CB911004,2011CB933504); 国家自然科学基金(81071870) |
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| 摘 要： | 目的构建MUC1靶向纳米粒,并在体外评估其抗肿瘤效应。方法 采用聚乳酸聚乙醇酸共聚物(PLGA)制备包载紫杉醇的纳米粒,并在表面偶联具有MUC1蛋白靶向性的核酸适配体。紫外分光光度法、动态光散射法测定MUC1靶向载药纳米粒的基本表征;以MUC1过表达的乳腺癌细胞MCF-7为实验组,肝癌细胞HepG2为对照组,用流式细胞仪检验纳米粒的选择性,MTS法评估其杀伤效果和IC50。结果 MUC1靶向载药纳米粒粒径(225.3±9.2)nm,药物包封率83.6%±1.7%,在体外可以特异性地被MUC1+癌细胞摄入,对MCF-7细胞杀伤作用显著强于普通载药纳米粒(P<0.01),IC50为1.52 mg/L。结论 该靶向纳米粒可以在体外特异性地增加MUC1+肿瘤细胞对纳米粒的摄入,降低IC50值,提高抗化疗药物对肿瘤细胞的杀伤效率。
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| 关 键 词： | MUC1 核酸适配体 靶向给药 肿瘤 |
Preparation of MUC1-targeted nanoparticles and evaluation of its cytotoxicity in vitro |
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| YU Chen-chen,HU Yan,DUAN Jin-hong,WANG Chen,XU Hai-yan,YANG Xian-da. Preparation of MUC1-targeted nanoparticles and evaluation of its cytotoxicity in vitro[J]. Basic Medical Sciences and Clinics, 2011, 31(7) |
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| Authors: | YU Chen-chen HU Yan DUAN Jin-hong WANG Chen XU Hai-yan YANG Xian-da |
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| Affiliation: | YU Chen-chen1,HU Yan1,DUAN Jin-hong1,WANG Chen2,XU Hai-yan1,YANG Xian-da1(1.Dept.of Physiology and Pathophysiology,Institute of Basic Medical Sciences,CAMS & PUMC,Beijing 100005,2.National Center ofNanoscience and Technology,Beijing 100190,China) |
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| Abstract: | Objective To construct MUC1-targeted nanoparticles and evaluate its cytotoxicity in vitro.MethodsMUC1 aptamers were conjugated to the surface of nanoparticles which were made of poly(lactic-co-glycolic-acid)(PLGA) nanoparticles and loaded with paclitaxel(PTX).The MUC1-targeted nanoparticles(Apt-NP-PTX) were characterized by UV spectrophotometry and dynamic light scattering.Using MUC1-overexpressing MCF-7 breast cancer cell as experimental group,and HepG2 as control group,the specificity of the Apt-NP-PTX wa... |
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| Keywords: | MUC1 aptamer targeted drug delivery tumor |
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