| Hax1基因及其特异性小干扰RNA对人胚肾293细胞凋亡的影响 |
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| 引用本文： | 龙琳,刘力. Hax1基因及其特异性小干扰RNA对人胚肾293细胞凋亡的影响[J]. 基础医学与临床, 2011, 0(6) |
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| 作者姓名： | 龙琳 刘力 |
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| 作者单位： | 中国医学科学院基础医学研究所;北京协和医学院基础学院病原生物学系; |
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| 基金项目： | 国家自然科学基金(30871283); 2010年中国医学科学院基础医学研究所所院长基金(2010PYZ17) |
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| 摘 要： | 目的构建Hax1基因特异性小干扰RNA(sihax),检测sihax对Hax1基因的表达抑制,在293细胞中研究Hax1和Hax1siRNAs对细胞凋亡的影响。方法设计Hax1siRNA序列构建真核表达载体并转染到293细胞,倒置荧光显微镜下观察、流式细胞仪、AnnexinV-EGFP/PI双染法及RT-PCR检测靶基因Hax1的表达,以及293细胞的凋亡。结果成功构建了Hax1siRNA干扰质粒pBS/U6-sihaxA和pBS/U6-sihaxB,并且sihaxB对Hax1抑制效应明显强于sihaxA。过量表达Hax1可显著抑制293细胞的早期凋亡比例。用sihaxA和sihaxB抑制内源性Hax1表达,可使晚期细胞凋亡比例从对照的9.03%±0.473%分别增加到10.8%±0.513%(P<0.05)和16.6%±0.858%(P<0.01)。与此同时,sihaxB还可使早期细胞凋亡比例由37.3%±0.5%降低到32%±1.77%(P<0.01)。结论 Hax1siRNA对靶基因Hax1的抑制作用与靶序列的保守性有一定相关性。Hax1基因在细胞中参与凋亡相关的多种调节过程。本研究为进一步探讨Hax1...
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| 关 键 词： | Hax1基因 小干扰RNA 293细胞 凋亡 |
The effect of Hax1gene and its specific small interference RNAs on the apoptosis of 293 cells |
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| LONG Lin,LIU Li. The effect of Hax1gene and its specific small interference RNAs on the apoptosis of 293 cells[J]. Basic Medical Sciences and Clinics, 2011, 0(6) |
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| Authors: | LONG Lin LIU Li |
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| Affiliation: | LONG Lin,LIU Li(Dept.of Pathogen Biology,Institute of Basic Medical Sciences,CAMS & School of Basic Medicine,PUMC,Beijing 100005,China) |
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| Abstract: | Objective To construct the Hax1 gene specific siRNA(sihax) for detection of sihax mediated Hax1 gene repression and to study the influence of Hax1 and Hax1 siRNAs on cell apoptosis.Methods The oligo sequences of Hax1 siRNAs were selected,and the constructed eukaryotic expression vectors were transfected into 293 cells.The influence of Hax1 and its siRNAs on cell apoptosis was evaluated by a number of approaches such as inverted fluorescence microscope,flow cytometric analysis,annexin V-EGFP/PI double staini... |
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| Keywords: | Hax1 siRNA 293 cells apoptosis |
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