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| MTHFR基因C677T多态性与冠心病患者血浆同型半胱氨酸和叶酸水平相关 | |
| 引用本文： | 胡小平,刘春莲,武玲,戴晓婧,彭亮,焦海燕,陈银涛.MTHFR基因C677T多态性与冠心病患者血浆同型半胱氨酸和叶酸水平相关[J].基础医学与临床,2011,31(7). |
| 作者姓名： | 胡小平 刘春莲 武玲 戴晓婧 彭亮 焦海燕 陈银涛 |
| 作者单位： | 1. 宁夏医科大学省部共建生育力保持教育部重点实验室,宁夏,银川,750004;宁夏医科大学医学遗传学与细胞生物学系,宁夏,银川,750004 2. 宁夏医科大学第一附属医院,宁夏,银川,750004 3. 日本东京大学,日本,东京,113-0033 |
| 基金项目： | 宁夏回族自治区自然科学基金项目(NZ0876) |
| 摘 要： | 目的研究宁夏地区汉族人群5,10-亚甲基四氢叶酸还原酶基因(MTHFR)C677T多态性、同型半胱氨酸水平(Hcy)及叶酸水平与冠心病(CHD)的相关性。方法用病例-对照研究方法、应用限制性片段长度多态性扩增技术(PCR-RFLP)分析宁夏地区汉族202例冠心病患者及199例正常人群MTHFRC677T基因型频率及基因频率的分布特点。荧光偏振免疫分析法测定血浆Hcy水平,化学发光免疫分析法测定血清叶酸、VitB12浓度。结果 (1)病例组与对照组MTHFRC677T基因型频率分别为CC型23.3%vs20.7%、CT型52.3%vs54.5%和TT型24.4%vs24.8%,两组间基因型及等位基因频率分布无差异。(2)冠心病患者组中MTHFR基因C677TCC基因型患者血浆Hcy浓度(10.84μmol/L)较T基因携带者(12.24μmol/L)低(P<0.01)。CC基因型患者血浆叶酸浓度(5.38μg/L)较T基因携带者(3.72μg/L)高(P<0.05)。结论 MTHFRC677T的3种基因型频率在宁夏汉族冠心病患者和正常人群中的分布无统计学意义。MTHFR基因C677T多态性与冠心病的危险因素Hc... |
| 关 键 词： | 冠状动脉疾病 5 10-亚甲基四氢叶酸还原酶 基因多态性 |
Association of methylenterahydrofolate reductase gene C677T polymorphism and plasma homocysteine and folate concentration with coronary heart disease |
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| HU Xiao-ping,LIU Chun-lian,WU Ling,DAI Xiao-jing,PENG Liang,JIAO Hai-yan,CHEN Yin-tao.Association of methylenterahydrofolate reductase gene C677T polymorphism and plasma homocysteine and folate concentration with coronary heart disease[J].Basic Medical Sciences and Clinics,2011,31(7). | |
| Authors: | HU Xiao-ping LIU Chun-lian WU Ling DAI Xiao-jing PENG Liang JIAO Hai-yan CHEN Yin-tao |
| Institution: | HU Xiao-ping1,2,LIU Chun-lian3,WU Ling3,DAI Xiao-jing4,PENG Liang1,JIAO Hai-yan1,CHEN Yin-tao1,2(1.Key Laboratory of Fertility Preservation and Maintenance(Ningxia Medical University),Ministry of Education,2.Dept.of Medical Geneticsand Cell Biology,3.the First Affiliated Hospital,Ningxia Medical University,Yinchuan 750004,China,4.Universityof Tokyo,Tokyo 113-0033,Japan) |
| Abstract: | Objective To investigate the correlation between methylenterahydrofolate reductase(MTHFR) gene C677T polymorphism,homocysteine(Hcy),folate and coronary heart disease(CHD) in the Han population of Ningxia region.Methods In this case-control study,we selected 202 patients with CHD and 199 healthy controls in Han population from Ningxia.The gene polymorphism of MTHFR C677T was detected by PCR-RFLP assay.Plasma Hcy concentrations were measured by fluorescence polarization immunoassay(FPIA) and serum folate and ... |
| Keywords: | coronary disease 5 10-methylenterahydrofolate reductase gene polymorphism |
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