| miR-16促进Neuro2a细胞的凋亡 |
| |
| 引用本文： | 刘畅,刘伟,王明,阴彬,袁建刚.miR-16促进Neuro2a细胞的凋亡[J].基础医学与临床,2011,31(7):762-766. |
| |
| 作者姓名： | 刘畅 刘伟 王明 阴彬 袁建刚 |
| |
| 作者单位： | 1. 中国医学科学院,基础医学研究所,北京协和医学院,基础学院,医学分子生物学国家重点实验室,北京,100005
2. 中国医学科学院,基础医学研究所,北京协和医学院,基础学院,医学分子生物学国家重点实验室,北京,100005;中国医学科学院,基础医学研究所,北京协和医学院,基础学院,解剖与组织胚胎学系,北京,100005 |
| |
| 基金项目： | 国家自然科学基金(31071203) |
| |
| 摘 要： | 目的 检测过表达miR-16对小鼠Neuro2a细胞周期和凋亡的影响,探讨miR-16在肿瘤发病过程中的可能机制.方法 构建能够在细胞中过表达miR-16的真核表达载体pcDNA3.1-miR-16,利用Real-time PCR验证了表达载体的过表达效应.应用pcDNA3.1-miR-16载体转染Neuro2a细胞,...
|
| 关 键 词： | miR-16 Neuro2a细胞 细胞周期 凋亡 肿瘤 |
Overexrpession of miR-16 promotes apoptosis in Neuro2a cells |
| |
| LIU Chang,LIU Wei,WANG Ming,YIN Bin,YUAN Jian-gang.Overexrpession of miR-16 promotes apoptosis in Neuro2a cells[J].Basic Medical Sciences and Clinics,2011,31(7):762-766. |
| |
| Authors: | LIU Chang LIU Wei WANG Ming YIN Bin YUAN Jian-gang |
| |
| Institution: | LIU Chang1,LIU Wei1,2,WANG Ming1,YIN Bin1,YUAN Jian-gang1(1.The National Laboratory of Medical Molecular Biology,2.Dept.of Anatomy and Histology,Institute of Basic Medical Sciences,Institute ofBasic Medical Sciences,CAMS & PUMC,Beijing 10005,China) |
| |
| Abstract: | Objective To investigate the role of miR-16 in cell cycle and apoptosis in Neuro2a cells and explore the possible function of miR-16 in tumor.Methods The overexpression plasmid pcDNA3.1-miR-16 of miR-16 was cloned and introduced into Neuro2a cells.Real-time PCR was chosen to test the overexpression of miR-16.Cell cytometry was used to study the role of miR-16 in cell cycle and apoptosis,and apoptosis cells were quantitatively examined by TUNEL.Results After tranfection of pcDNA3.1-miR-16,the mature miR-16 w... |
| |
| Keywords: | miR-16 Neuro2a cells cell cycle apoptosis tumor |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
