5-FU乙醇脂质体制备及局部植入治疗家兔喉气管狭窄的效果分析

目的:评价5-氟尿嘧啶(5-FU)脂质体对家兔瘢痕性喉气管狭窄的治疗效果。方法:利用薄膜分散法制备5-FU乙醇脂质体,并检测脂质体形态、包封率等特性。采用刮擦法制备喉气管狭窄模型,环形刮除气管黏膜约0.5cm宽度,诱导气管内瘢痕形成,将诱导瘢痕性喉气管狭窄成功的模型动物随机分为3组:5-FU乙醇脂质体组(A组)、5-FU水溶液组(B组)和生理盐水组(C组),各组分别在内镜监视下经皮穿刺将药物注射入瘢痕内部,并记录此时狭窄度,记为0d,其后,在用药后7、14、21d纤维喉镜下观察记录气管狭窄情况。结果:治疗初期A组和B组并无差别,21d时A组狭窄度明显小于B组,并在观察期间没有出现再狭窄。结论:5-FU脂质体对家兔瘢痕型喉气管狭窄的治疗具有较好的效果,可以作为喉气管狭窄的治疗方法进行进一步研究。

Preparation of Ethosomes encapsulated with 5-fluorouracil and the effect of local administered 5-FU ethosomeon on laryngotracheal stenosis of rabbit

Objective:To evaluate the efficacy of Ethosomes encapsulated with 5-FU in treatment of laryngotracheal stenosis in rabbit models.Method:The 5-FU ethosome was prepared by the thin film hydration method,and the size distribution and the encapsulation efficiency was investigated.The tracheal mucosa was scraped about 0.5 cm in width with a nylon brush to induce the scar formation in the airway,then animals were divided into three groups:5-FU ethosome group,5-FU group and saline group.Drugs were injected into scar by paracentesis under endoscope in each group respectively.The severity of stenosis was observed under laryngofiberoscope immediately,7,14,21 days after administration.Result:Airway stenosis of 5-FU ethosome group was not significantly different compared with 5-FU group at 7 days after administration,but 5-FU ethosome significantly reduced the airway stenosis at 21 days after administration when compared with 5-FU group and no restenosis was noticed during the observation period.Conclusion:Ethosomes encapsulated with 5-FU was effective for laryngotracheal stenosis.It is a ptentially new method for ameliorating airway stenosis originated from granulation tissue.