葡萄子原花青素对糖尿病大鼠主动脉载脂蛋白A-Ⅰ表达的影响

目的 观察葡萄子原花青素(GSPE) 对链脲佐菌素(STZ) 诱导的糖尿病大鼠主动脉载脂蛋白A-Ⅰ（apoA-Ⅰ）的影响。方法 雄性Wistar 大鼠随机分成对照组（C组，n=10）、糖尿病组(DM组,n=9) 和糖尿病GSPE 治疗组(DM+GSPE组,n=13)。24周后处死动物，留取主动脉标本，HE染色观察各组动物主动脉形态变化，并应用荧光差异凝胶电泳技术观察GSPE对糖尿病大鼠主动脉apoA-Ⅰ表达的影响。结果 糖尿病组大鼠主动脉内皮细胞肥大，结构紊乱，平滑肌细胞增生，内弹力板破坏，管腔变小，经GSPE治疗后，上述变化均未出现，血管结构接近对照组。糖尿病组大鼠apoA-Ⅰ表达较对照组明显下降（DM组/C组：-1.68），而应用GSPE治疗后apoA-Ⅰ表达上调（DM+GSPE组/DM组：1.77）。结论 GSPE能够促进STZ诱导的糖尿病大鼠主动脉apoA-Ⅰ表达，该机制可能与GSPE的血管保护作用相关。

Effect of grape seed proanthocyanidin extracts on expression of aortic apolipoprotein A- in diabetic rats

Objective To explore the effect of grape seed proanthocyanidin extracts (GSPE) on expression of aortic apolipoprotein A-Ⅰ(apoA-Ⅰ) in streptozotocin(STZ) induced diabetic rats. Methods Male Wistar rats were randomly divided into the control group (group C, n=10), the diabetic group (group DM, n=9) and the GSPE treatment diabetic group (group DM+GSPE, n=13). All animals were sacrificed after 24 weeks, and the aortas were excised about 1cm from the end, fixed in 10% formaldehyde, embedded in paraffin and cut into 4μm-thick sections for light microscopy. Then they were stained with hematoxylin-eosin under a light microscope at a magnification of 200×. In addition, difference gel electrophoresis technique was used to analyze the effect of GSPE on expression of aortic apoA-Ⅰ. Results In diabetic rats, hypertrophy and disarray of endothelial cells occurred, and proliferation of smooth muscle cells were observed in the aortic wall, and the internal elastic laminae were impaired. The lumina of the aorta became smaller. After treatment of GSPE, light microscopic findings were similar to those of the control rats. Expression of aortic apoA-Ⅰwas the lowest in the diabetic group（DM group/C group：-1.68）, but after treatment with GSPE, it was up-regulated（DM+GSPE group/DM group：1.77）. Conclusion GSPE can promote expression of aortic apoA-Ⅰ in diabetic rats, which may play a role in the angio-protective function of GSPE.