| 逆转录病毒载体携带报告基因在晶状体上皮细胞中的转染与表达 |
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| 引用本文： | 万光明,张效房.逆转录病毒载体携带报告基因在晶状体上皮细胞中的转染与表达[J].眼科研究,2004,22(1):34-36. |
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| 作者姓名： | 万光明 张效房 |
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| 作者单位： | 450052,郑州大学第一附属医院眼科 |
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| 基金项目： | 河南省自然科学基金(20004023300)，国家博士后基金(10059)资助 |
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| 摘 要： | 目的观察目的基因在晶状体上皮细胞的表达情况，以探讨基因疗法用于后发性白内障的可行性。方法用绿色荧光蛋白基因作为报告基因，以逆转录病毒载体携之转染晶状体上皮细胞，观察GFP在晶状体上皮细胞的表达情况。结果基因转染后可见晶状体上皮细胞的GFP表达率在30％～35％。结论逆转录病毒载体可以携带目的基因转染于晶状体上皮细胞，并达到较高的转染率。
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| 关 键 词： | 逆转录病毒载体 报告基因 晶状体上皮细胞 转染 表达 基因治疗 |
Retrovirus-mediated transfer of GFP gene into lens epithelial cells in vitro |
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| Wan Guangming,Zhang Xiaofang.Retrovirus-mediated transfer of GFP gene into lens epithelial cells in vitro[J].Chinese Ophthalmic Research,2004,22(1):34-36. |
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| Authors: | Wan Guangming Zhang Xiaofang |
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| Institution: | Wan Guangming,Zhang Xiaofang.Department of Ophthalmology,First Affiliate d Hospital,Zhangzhou University,Zhengzhou450052,China |
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| Abstract: | Object iv e To study the possibility of prevention of posterior capsular opacification(PC O)by transfer of exogenous gene into lens epithelial cells.Methods Green fluo rescence protein(GFP)gene was used as a reporter gene,and was transferred int o lens epithelial cells mediated by retroviral vector,GFP expression in cells w as analyzed.Results The positive GFP gene was about 30%-35%in lens epith elial cells.Conclusion Retrovirus-mediated gene may be the therapeutically val uable for the treatment of posterior capsular opacification. |
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| Keywords: | lens epithelial cells posterior capsular opacification reporter gene green fluorescence protein gene therapy |
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