BACTEC MGIT960系统快速培养分枝杆菌的效果评价

目的与传统罗氏培养法相比较，评价BACTECMGr／960快速培养系统在基层结控实验室的应用效果及前景。方法采用改良罗氏培养基、BACTECMGIT960液体培养基对521份临床痰标本进行平行接种培养，比较两者的阳性率、培阳时间、污染率。结果传统罗氏培养法的阳性率为47．1％（81／172），BACTECMGIT960系统的阳性率为68．0％（117／172）；传统罗氏培养法最早培阳时间为12d，平均26d，BACTECMGr1960快培系统最早培阳时间为4d，平均12d；传统罗氏培养法污染率为2．5％（13／521），BACTECMGr1960快培系统的污染率为3．5％（18／521）；结论BACTECMGIT960系统可以提高分枝杆菌的培养阳性率和明显缩短培养时间，污染率与传统罗氏培养相较无显著性差异，是一种快速，有效的检测方法，对基层结控机构早期发现肺结核病人和疗效观察将发挥重要作用。

Evaluation of BACTEC MGIT960:a new system for rapid mycobacterium culture

Objective Comparing BACTEC MGIT960: a mycobacterium rapid culture system with the traditional L-J culture,assessing its application and prospect.Method By L-J medium and BACTEC MGIT960 liquid medium,521 clinical sputum specimens were inoculated in parallel culture,comparing the positive rate,contamination rate,average growth time,negative reporting time.Result The culture positive rate of BACTEC MGIT960 system and L-J traditional method were 68.0%(117/172)and 47.1%(81/172) First growth time of BACTEC MGIT96...