肺结核患者外周血记忆性T细胞及B细胞亚群的检测分析

目的探讨肺结核患者外周血记忆性T及B细胞亚群的表达水平。方法采用流式细胞仪检测肺结核患者（TB）、潜伏感染者（LTBI）、健康人（HD）外周血记忆性T、B细胞亚群水平及B细胞表达水平；利用Elispot技术检测外周血单个核细胞（PBMC）结核菌抗原特异性IFN-r分泌水平。结果流式结果显示，TB及LTB1的CD4＊及CD8＊中心记忆性T细胞（Tcm）显著增多，以CD4＊尤为显著；相反，效应记忆性T细胞（Tem）减少。TB记忆性B细胞均低于HD和LTBI（P〈0．01）；而在HD与LTBI及TB这三组CD19＆细胞占淋巴细胞比例中都有明显差异（P〈0．01；P〈0．001）。CD4’／CD8’Tcm与IFN-γ ELISPOT结果成正相关。结论肺结核患者外周血中存在着记忆性T及B细胞亚群的紊乱，中心记忆性T细胞增多，效应记忆性T细胞减少，记忆性T、B细胞的数量及免疫状态与结核菌感染的不同转归有关，记忆性T、B细胞在结核病发病机制中发挥着重要作用。

Analysis of memory T cell and B cell subsets in the peripheral blood of patients with pulmonary tuberculosis

Objective To study the expression of memory T cell and B cell subsets of peripheral blood in patients with pulmonary tuberculosis. Methods The memory T cell and B cell subsets of peripheral blood were analyzing with flow cytomety ; Antigen specific-IFN-γ response were determined in PBMCs by using in-house Elispot. Results CD4＋ and CD8＋ central memory T cells in TB and LTBI were much higher ,especially CD4＋ . On the contrary, effective memory T cells were reduced. Memory B ceils in TB were decreased compared with HD and LTBI. （ P 〈0. 01 ,P 〈0. 001 ）. The proportion of CD4 ,CD8＋ central memory T ceils is signi？ cantly correlated with the released levels of specific IFN-γ. Conclusion Memory T and B cell subsets were confused in peripheral blood of patients with pulmonary tuberculosis, Central memory T cell increased, while effort memory T cell decreased ; the population of memory T/B cells and immu- nization were associated with the result of Mycobacterium tuberculosis infection. Memory T and B cells play an important role in the tuberculosis mechanism.