喉鳞状细胞癌组织中p63表达的临床意义

目的：探讨p63基因在喉鳞状细胞癌中的表达及其临床意义。方法：用免疫组织化学法检测63例喉鳞状细胞癌（喉癌组）及其45例癌旁组织（癌旁组）、24例转移淋巴结（转移淋巴结组）、40例未转移淋巴结（未转移淋巴结组）及25例喉非癌组织（非喉癌组）中p63基因的表达。结果：p63基因在喉鳞状细胞癌组织中均为细胞核阳性表达，喉癌组及转移淋巴结组的中、强阳性率为95．2％（60／63）和83．3％（20／24）；癌旁组及未转移淋巴结组中无中、强阳性表达；非喉癌组中1例乳头状瘤为弱阳性表达，其余均为上皮细胞或基底细胞核弱阳性表达。结论：p63可作为喉鳞状细胞癌有特异性的标记物，对喉鳞状细胞癌的早期诊断和鉴别诊断有重要意义。p63可能主动参与了调控喉鳞状细胞癌的发生过程，但在喉鳞状细胞癌的发展过程中处于被激活状态，可能在发展过程中发挥负反馈的作用。

The clinical significance of the expression of p63 gene in laryngeal squamous cell carcinoma

OBJECTIVE: To study the expression and clinical significance of p63 gene in laryngeal squamous cell carcinoma(LSCC). METHOD: The immunohistchemical technique was used to detected the expression of p63 in 63 cases of LSCC, 45 cases of para-cancer tissues, 24 cases of lymph node metastasis, 40 cases of non lymph node metastasis and 25 normal tissues. RESULT: The positive overexpression rate of the p63 gene in LSCC (95.24%) and in matched metastastic lymph node (83.3%) was significantly higher than para cancer tissues, non metastasis lymph node and normal tissues (P<0.01). p63 gene expression was not correlation with the clinical stages,clinical typing and pathological classification of the LSCC(P >0.05). CONCLUSION: p63 gene had important clinic significance to the diagnosis and differential diagnosis of the LSCC. It perhaps participate in regulation of tumorigenesis in LSCC, and it may play negative feedback action in the development of LSCC.