| 实时荧光定量PCR在念珠菌研究中的应用 |
| |
| 引用本文： | 张云操,白丽.实时荧光定量PCR在念珠菌研究中的应用[J].中国热带医学,2010,10(2):241-243. |
| |
| 作者姓名： | 张云操 白丽 |
| |
| 作者单位： | 大理学院基础医学院医学微生物与免疫学教研室,云南,大理,671000 |
| |
| 基金项目： | 大理学院高层次人才基金(No.KY430440) |
| |
| 摘 要： | 念珠菌作为院内感染的常见机会性致病菌,实时荧光定量PCR技术已应用于念珠菌临床快速诊断、基因分型、耐药等研究。实时荧光定量PCR技术以灵敏、快速、安全、准确、高通量等特性,不仅在分子生物学领域作为常规的分析检测方法,而且在医院临床标本病原体的检测、遗传性疾病的诊断、检疫、法医学标本的鉴定等领域也逐步得到了广泛运用。
|
| 关 键 词： | 实时荧光定量PCR（RTFQ—PCR） 念珠菌 |
Application of real-time fluorescent quantitative PCR in research of Candida species |
| |
| ZHANG Yun-cao,BAI Li.Application of real-time fluorescent quantitative PCR in research of Candida species[J].China Tropical Medicine,2010,10(2):241-243. |
| |
| Authors: | ZHANG Yun-cao BAI Li |
| |
| Institution: | ZHANG Yun-cao,BAI Li. (Department of Medical Microbiology and Immunology,School of Basic Medicine,Dali University,Dali 671000,Yunnan,P. R. China) |
| |
| Abstract: | Candida species is the commone opportunist pathogen responsible for candidiasis in hospital infection.In this paper we reviewed the application of RTFQ-PCR in dentification,drug resistance,genotypic identification of Candida species were summerized in detail. RTFQ-PCR is an advanced medium that permits the expression of highly specific,rapid,proven, accurate,high-flux method. At present,different applications of it emerge rather frequently and widely,not noly in areas with molecular biology for standard eva... |
| |
| Keywords: | Real-time fluorescent quantitative PCR(RTFQ-PCR) Candida species |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
