养胃解毒合剂对脓毒症模型大鼠作用的实验研究

目的观察养胃解毒合剂对脓毒症模型大鼠的治疗作用，探索养胃解毒合剂治疗脓毒症的部分作用机制。方法60只SD大鼠随机分为假手术组、脓毒症模型组、养胃解毒合剂组、阿莫西林组。采用盲肠结扎穿孔法对大鼠进行脓毒症模型的复制。假手术组大鼠不进行穿孔。灌胃相应药物干预．连续给药6次。采用酶联免疫吸附（ELISA）法对各组大鼠血清中内毒素、肿瘤坏死因子-γ（TNF-γ）、干扰素-γ（INF-γ）的含量进行检测。结果模型组大鼠血清中内毒素、TNF—α、IFN-γ的含量显著高于假手术组（P〈0．01）；养胃解毒合剂组大鼠血清中内毒素、TNF—α、IFN-γ的含量明显低于脓毒症模型组（P〈0．01）。结论养胃解毒合剂能够明显降低脓毒症模型大鼠血清中内毒素、TNF-α及IFN-γ的含量。

Experimental Study of Yangweijiedu Mixture for Sepsis in Rats

Objective： To observe the effect of Yangwei Jiedu Decoction on sepsis model in rats and explore the mechanism. Methods： 60 SD rats were randomly divided into four groups,respectively,for the sham operation group （group A ）, sepsis （CLP） model group （group B ）, Yangwei Jiedu Decoction treatment group （group C ）, control group（group D） of amoxieillin. Using a cecal ligation and perforation method for B ,C ,D three groups of rats were sepsis model replication,the rats in group A operation with more than three groups, but without perforation. After oral administration of 8h a corresponding drug intervention, group B received saline by oral garage,continuous dosing 6 times. The use of enzyme-linked immunosorbent assay （ELISA） method on serum endotoxin （endotox- in） ,tumor necrosis factoro-α （TNF-α） ,IFN-γ （INF-γ） was detected. Measurement data were confirmed by SPSSIO.O software to carry on statistical analysis. Results： In serum of model rats with endotoxin,TNF-α,IFN- γ was significantly higher than that in sham operation group（P 〈 0.01 ） ;stomach Jiedu Decoction group in serum of rats with endotoxin ,TNF-α ,IFN-γ levels were significantly lower in sepsis model group （P〈0.01）. Conclusion： Stomach Jiedu Decoction can significantly reduce sepsis model in rats serum endotoxin,tumor necrosis factorα and IFN-γcontent.