抗HIV药物的筛选评价方法

随着对艾滋病致病机制的深入研究，越来越多新的抗艾滋病病毒（HIV）药物进入临床试验，但大量抗HIV药物的应用也导致了耐药性问题的出现。因此，建立高通量快速的抗HIV药物筛选平台对研究新的抗HIV药物非常重要。本文综述了目前抗HIV药物研究中常用的体外和体内筛选模型，重点阐述了体外以病毒复制特异性功能活动或特定结构作为靶点的筛选系统及其评价方法。

Screening and evaluating system of anti-HIV drugs

With understanding of mechanism of HIV infection, more and more new anti-HIV agents are developed and used in clinical experiments. However, the abuse of anti-HIV drugs was causing serious drug-resistance problem, so it is very important to develop new high-throughput screening system for drug discovery. In this review the commonly used in vitro and in vivo anti-HIV agents screening models are outlined, particularly the in vitro screening system targeting the specific activity or protein structure in viral production.