Structural proteins of Marek's disease virus |
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| Authors: | J H Chen L F Lee K Nazerian B R Burmester |
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| Affiliation: | 4 United States Department of Agriculture Regional Poultry Research Laboratory, ARS East Lansing, Michigan 48823 USA;5 Department of Microbiology and Public Health, Michigan State University, East Lansing, Michigan 48823 USA |
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| Abstract: | Marek's disease virus (MDV) was propagated in roller-bottle cultures of duck embryo fibroblasts and partially purified by sucrose gradient centrifugation. Analysis of viral protein by polyacrylamide gel electrophoresis revealed that at least eight proteins (designated VPI-VPVIII) were present in MDV. The VPI is the major viral protein. At least two viral proteins, VPII and VPIV, with glucosamine label could be detected. These two peaks may represent viral glycoprotein and may be associated with the viral envelope. No host cell proteins coelectrophoresed with any viral proteins. Similar electropherograms were obtained by coelectrophoresis of MDV and herpes simplex virus proteins and of MDV and pseudorabies virus proteins. |
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