| HPLC-ELSD法测定雷公藤提取物中雷公藤甲素的含量 |
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| 引用本文： | 姜佳峰,朱丽华,张理星.HPLC-ELSD法测定雷公藤提取物中雷公藤甲素的含量[J].齐鲁药事,2004,23(1):29-30. |
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| 作者姓名： | 姜佳峰 朱丽华 张理星 |
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| 作者单位： | 1. 华中科技大学,武汉,430074
2. 鲁南制药厂,临沂,276003 |
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| 摘 要： | 目的 :HP -ELSD法测定雷公藤甲素。方法 :alltech -CN色谱柱 (4 6× 5 0mm ,5 μm) :流动相 :甲醇 -水 (4 5∶5 5 ) ;流速 :0 6mL/min ;检测器漂移管温度 :95℃ ;气流速 :2 5L/min。结果 :雷公藤甲素在 0 1～ 1 5 μg范围内线性关系良好 ,加样回收率为 98 4 % (n =9)。结论 :该方法简便、准确 ,稳定性好 ,线性范围宽。可作为雷公藤甲素的含量测定方法
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| 关 键 词： | LC-ELSD 雷公藤 雷公藤甲素 |
| 文章编号： | 1672-7738(2004)01-0029-02 |
Determination of Triptolide in Tripterygium Wilfordii Hook f.ext.with HPLC-ELSD |
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| Jiang Jia-feng,Zhu Li-hua and Zhang Li-xing.Determination of Triptolide in Tripterygium Wilfordii Hook f.ext.with HPLC-ELSD[J].qilu pharmaceutical affairs,2004,23(1):29-30. |
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| Authors: | Jiang Jia-feng Zhu Li-hua and Zhang Li-xing |
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| Institution: | Jiang Jia-feng,Zhu Li-hua and Zhang Li-xing 1 |
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| Abstract: | The content of Triptolide in Tripterygium Wilfordii was determined by HPLC-ELSD.Parameters were set as the follow : alltech-CN column ( 4.6×250mm , 5μm ) ; methanol-water ( 45:55 ) as the eluent , flow rate 0.6 mL/min ; inspector temperature 95℃ ; gas flow rate 2.5 L/min.results showed that Triptolide had good linear relation during 0.1-1.5μg , the recoveries of added constituents was 98.4% ( n=9 ).This method was convenient , correct , stable , wide in linearity range and suitable for determination of Triptolide content. |
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| Keywords: | HPLC-ELSD Tripterygium Wilfordii Triptolide |
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