日本血吸虫可溶性成虫抗原和虫卵抗原诱导调节性T细胞能力差异的比较

目的 目的 观察并比较日本血吸虫可溶性成虫抗原 （SWA） 与可溶性虫卵抗原 （SEA） 诱导CD4+ CD25+ Foxp3+ 调节性T （Treg） 细胞水平及其抑制能力的差异。方法 方法 分别以PBS、 SWA和SEA免疫小鼠， 取小鼠脾淋巴细胞， 采用流式细胞术检测各组Treg细胞占CD4+ T细胞的比例， 以及Treg细胞中产IL?10、 TGF?β的细胞比例。采用磁珠分选出各组小鼠的Treg细胞后， 以3 H?TdR掺入法检测其抑制靶细胞增殖的能力。结果 结果 与SWA相比， SEA可诱导更多的Treg细胞 （P < 0.05）， 刺激 Treg细胞免疫抑制功能的能力也更强 （P < 0.05）； SWA、 SEA免疫小鼠后， SEA刺激Treg细胞产生IL?10、 TGF?β的能力更强（P < 0.01）。结论 结论 SEA较SWA能更好地活化诱导Treg细胞发挥免疫抑制作用。

Activities of Treg cells stimulated by soluble adult worm antigen and egg antigen of Schistosoma japonicum

Objective To observe and compare the effects of soluble adult worm antigen（SWA）and soluble egg antigen （SEA）of Schistosoma japonicum on the induction of Treg cells and the suppressive activity of Treg cells. Method Splenocytes were prepared from mice treated with PBS，SWA，and SEA，respectively，and then the proportions of Treg cells and the levels of IL?10 and TGF?β in Treg cells were determined by FACS. The purified Treg cells from the mice treated as above?mentioned were detected for their immunosuppressive activities by incorporation of［3 H］thymidine for the final 16 h of culture. Results Com? pared to SWA，SEA induced the higher proportion of Treg cells with a stronger suppressive activity，which produced the higher levels of IL?10 and TGF?β（P < 0.05） . Conclusion SEA significantly induces Treg cells and enhances their immunosuppressive activity.