HBV携带者血清和白带HBV-DNA FQ-PCR检测分析

目的了解乙型肝炎病毒HBV携带者血清与白带中HBV—DNA含量的关系及其意义。方法选择经血清学检测诊断为女性HBV携带者867例，采用荧光定量聚合酶链反应（FQ—Pen）技术测定其白带中和血清HBV—DVA含量，并对所有检测指标进行相关性分析。结果867例携带HBV女性中，HbsAg、HbeAg、HbcAb三大阳者的血清和白带中HBV—DNA的阳性检出率均为100％；HbsAg、HbeAb、HbcAb三小阳组阳性率分别为85．6％和84．3％；HB．sAg、HBsAb两组的阳性率分别为86．6％和83，7％，白带HBV—DNA含量与血清HBV—DNA含量呈正相关（r=0，896），且白带HBV—DNA含量低于血清含量近10^1。蛄论白带与血清中HBV—DNA含量呈正相关。血清HBV—DVA阳性的育龄女性应作白带HBV—DVA检测，以对其阳性者采取防治措施，降低HBV母婴播率具有重要意义重大。

Detection and analysis of HBV-DNA from serum and whites in HBV carriers by FQ-PCR

Objective To analyze the content of HBV-DNA from serum and whites of HBV carriers and its significance. Methods The content of HBV-DNA in the serum and whites of 867 female HBV carriers confirmed by serological test was detected by FG-PCR and the results were analyzed. Results The detection rates of HBV-DNA in serum and whites of 867 female HBV carriers all positive for HBsAg, HBeAg and HBcAb were 100%. While the detection rates of HBV-DNA in the HBV carriers positive for HBsAg, HBeAb and HBcAb were 85.6% and 83.4%. The detection rates of HBv-dna in the those positive for HBsAg and HBcAb were 86.6% and 83.4%. The HBV-DNA content in whites was positive correlated to that in serum (r=0.896). Conclusion The content of HBV-DNA in the whites is positive correlated to the content of HBV-DNA in serum of female HBV carriers. HBV-DNA be detected from the whites of female HBV carriers positive for HBV-DNA in serum. Measures be taken to reduce the vertical transmission of hepatitis B from mothers to babies.