| 阳离子交换树脂分离-高效液相色谱法测定奥沙利铂脂质体包封率 |
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| 引用本文： | 孙中英,吴小涛,张自强,李战,张朝晖. 阳离子交换树脂分离-高效液相色谱法测定奥沙利铂脂质体包封率[J]. 西北药学杂志, 2009, 24(5): 382-384 |
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| 作者姓名： | 孙中英 吴小涛 张自强 李战 张朝晖 |
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| 作者单位： | 1. 江苏大学药学院中药学系,江苏,镇江,212013
2. 南京长澳医药科技有限公司,江苏,南京,210038 |
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| 摘 要： | 目的建立奥沙利铂脂质体包封率的测定方法。方法用阳离子交换树脂分离游离药物和被包封药物,采用高效液相色谱法测定奥沙利铂含量,计算包封率。结果阳离子树脂对溶液中游离奥沙利铂的吸附率大于93%,对载药后的脂质体无吸附;HPLC含量测定方法的线性范围为1～100mg·L-1(R2=0.9999),精密度RSD<2%。结论阳离子树脂-HPLC法简便快捷,成本较低,且无空白干扰,方法学稳定,适用于奥沙利铂脂质体包封率的测定。
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| 关 键 词： | 奥沙利铂 脂质体 阳离子树脂 包封率 HPLC |
Determination of the entrapment efficiency of oxaliplatin-liposomes by cationic exchange resin-HPLC |
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| Abstract: | Objective To establish a method for determining the entrapement of oxaliplation liposomes;Method The free oxaliplatin and its liposomes were separated by using cationic exchange resin,and the concentration of oxaliplatin was determined by HPLC;Result Cationic exchange resin's absorption rate of oxaliplation in solutions was more than 93%,and cationic exchange resin had no absorption of the liposome loading oxaliplation. The linearity range of assaying was 1~100 mg·L-1,the precision evaluated by RSD was Less than 2%;Conclusion The established method for determination the entrapement efficiency of oxaliplatin liposomes is simple,rapid and cheap. |
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| Keywords: | HPLC |
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