Light microscopic demonstration of the microlumen of ependymoma: A study of the usefulness of antigen retrieval for epithelial membrane antigen (EMA) immunostaining |
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Authors: | Email author" target="_blank">Nobuyuki?KawanoEmail author Yoshie?Yasui Satoshi?Utsuki Hidehiro?Oka Kiyotaka?Fuji Shohei?Yamashina |
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Institution: | (1) Isobe Clinic, 941 Isobe, 228-0827 Sagamihara, Kanagawa, Japan;(2) Department of Neurosurgery, Kitasato University School of Medicine, Sagamihara, Japan;(3) Department of Anatomy, Kitasato University School of Medicine, Sagamihara, Japan |
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Abstract: | To determine the origin of dotlike epithelial membrane antigen (EMA) immunoreactivity of ependymoma, which is consistent with
the eosinophilic globular body in hematoxylin and eosin (H&E) stain, an immuno-electron microscopic study was undertaken.
The usefulness of antigen retrieval pretreatment in detecting the dotlike EMA immunoreactivity in ependymomas was also studied.
The materials were 29 ependymomas, 7 autopsy brains as a normal control, and 50 brain tumors of various types. The study confirmed
that most of the brown dots in EMA immunostain in ependymoma represented microlumina of tumor cells. In ependymomas, plain
EMA immunostaining showed dotlike positivity in only six cases (21%), and antigen retrieval pretreatment increased the number
of positives up to 26 cases (90%). Antigen retrieved CD99 detected 23 positive cases (80%) in ependymomas. On the basis of
the results, although some false positive findings were raised by antigen retrieval pretreatment, the authors positively recommend
adoption of the technique, especially when ependymoma remains as one of the differential diagnoses of the tumor. |
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Keywords: | Ependymoma EMA CD99 (MIC2) Immunostain Antigen retrieval |
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