膀胱移行细胞癌Runx3基因的甲基化

目的探讨Runx3基因甲基化与膀胱移行细胞癌的关系。方法采用甲基化特异性聚合酶链反应(MSP)检测76例配对膀胱移行细胞癌组织、癌旁正常黏膜组织中Runx3基因甲基化的情况。结果74%的膀胱移行细胞癌组织中存在Runx3基因异常甲基化,而相应的癌旁正常组织中该基因的甲基化率分别为14%。癌组织中Runx3基因甲基化的发生率显著高于癌旁正常组织(P<0.05)。该基因甲基化与肿瘤数目、分化程度、浸润深度等临床病理特征无关。结论Runx3基因异常甲基化是膀胱移行细胞癌发生、发展过程中的频繁事件,可作为膀胱移行细胞癌早期诊断的分子标记物。

Methylation status of Runx3 gene in human bladder transitional cell carcinoma

Objective To investigate the relationship between the methylation status of Runx3 gene and bladder transitional cell carcinoma.Methods The methylation status of Runx3 gene in 76 specimens of bladder transitional cell carcinoma and the corresponding normal bladder mucosa was detected by methylation-specific polymerase chain reaction (MS-PCR).Results Aberrant methylation of Runx3 gene was detected in 74% of bladder transitional cell carcinoma specimens and 14% of the corresponding normal mucosa,there were significant differences between the former and the latter (P<0.05).Runx3 methylation was not significantly correlated with the tumor number,differentiation degree,invasion depth of bladder transitional cell carcinoma.Conclusion Aberrant methylation of Runx3 gene is a common event in the occurrence and progression of bladder transitional cell carcinoma,which may be utilized as a molecular diagnostic marker for bladder transitional cell carcinoma.