Methylation Pattern of the CD14 and TLR2 Genes in Human Dental Pulp |
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| Authors: | Fabiano Pereira Cardoso,Simone Angé lica de Faria Amormino,Walderez Ornelas Dutra,Antô nio Paulino Ribeiro Sobrinho,Paula Rocha Moreira |
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| Affiliation: | ∗ Department of Restorative Dentistry, Faculdade de Odontologia, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil;† Department of Morphology, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil |
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| Abstract: | IntroductionPattern recognition receptors, such as toll-like receptor 2 (TLR-2) and TLR-4, participate in the activation of immune cells by microorganisms in dental pulp. However, the expression levels of pattern recognition receptors can be modulated by epigenetic factors, especially DNA methylation. In this study, the methylation status of the TLR-2 and CD14 (TLR4 co-receptor) genes in healthy and inflamed human dental pulp was examined.MethodsThe Methyl-Profiler DNA Methylation qPCR Assay was used to verify the DNA methylation patterns.ResultsNo differences in the methylation patterns were observed between the 2 groups. Most DNA was unmethylated in both groups.ConclusionsThe hypomethylation of TLR2 and CD14 genes is a usual feature in human dental pulp. |
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| Keywords: | CD14 dental pulp DNA methylation TLR2 |
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