应用单克隆抗体检测脑囊虫病患者的循环抗原

目的 评价单克隆抗体检测循环抗原(CAg)诊断脑囊虫病的价值。方法用猪囊尾蚴囊液抗原制备单克隆抗体486，以ELISA方法检测患者和对照者血清和／或脑脊液中的猪囊虫抗原。结果82例脑囊虫病患者血清CAg阳性率为79．2％(65／82)，腩脊液CAg阳性率为100％(26／26)，治疗一个疗程后血清CAg转阴率为85％(17／20)。从对照者标本中未检出猪囊虫CAg。486和包虫抗原有轻微交叉反应。结论CAg检测，尤其是脑脊液CAg检测有助于脑囊虫病的诊断，而血清CAg检测则有助于疗效考核。

Determination of Circulating Antigen in Neurocysticercosis Patients by Monoclonal Antibody

Objective To detect circulating antigen(CAg )for diagnosing neurocysticercosis.Method ELISA was performed with monoclonal antib ody 4B6against the cyst fluid antige n of Cyticercus cellulosae for detecting CAg in serum and /or cerebrospinal fluid(CSF)of patients with neurocysticercosis or with other diseases.Results In the group of 82cases of neurocysticercosis,the positive rate of serum CAg was 79.2%(65/82)and the positive rate of CSF CAg was100%(26/26).After chemotherapy for 20cases wit h positive serum CAg,the titer of serum CAg in 17cases dropped to zero(85%).CAg could not be detected in specime ns from patients with other diseases.Conclusion These results indicate that the determination of C Ag,especially of the CSF CAg,is useful for the diagnosis of neurocystice rcosis and the drop in serum CAg is a good para meter for the evaluation of the effectiveness of chemotherapy.